PhD Scientific Days 2017

Oral Presentations: Oncology

Role of the gut-liver axis in accelerated liver regeneration

Előadó neve

Tihanyi, Dóra Krisztina

Előadó munkahelye

Semmelweis University, 1st Department of Surgery

Előadó telefonszáma

+36305187139

Előadó e-mail címe

tihanyi.dora.krisztina@gmail.com

Az előadás címe

Role of the gut-liver axis in accelerated liver regeneration

Szerző(k) neve és munkahelye

D.K. Tihanyi1, A. Budai1, T. Kovacs1, A. Fulop1, T.M. van Gulik2, P. Olthof2, A. Szijarto1
1 Semmelweis University, 1st Deparment of Surgery, Budapest, Hungary
2 Academisch Medisch Centrum, Department of Experimental Surgery; Amsterdam, Netherlands

Szekció

Oral Presentations: Oncology

Data of the presenter

Doctoral School: Clinical Medicine
Program: Clinical and Experimental Research in Angiology
Supervisor: Attila Szijártó
E-mail: tihanyi.dora.krisztina@gmail.com

Text of the abstract

Background: Associating liver partition and portal vein ligation for staged hepatectomy (ALPPS) is a novel method to induce more rapid liver hypertrophy compared to portal vein occlusion techniques. Despite the major intest in induced rapid hypertrophy, the exact mechanisms behind this phenomenon are still unknown. Aim of this study was to investigate the role of gut-liver axis in portal vein ligation (PVL) and ALPPS induced liver hypertrophy.
Material & Methods: Male Wistar rats underwent PVL and ALPPS (n=60). Before the surgery and after 24h, 48h, 72h, 168h the portal pressure and the hepatic lobes weight were measured. We investigated the mRNA expression of FXR (Farnesoid-X Receptor) and FGFR4 (Fibroblast Growth Factor Receptor 4) of the regenerated lobe and the ileum. The portal and the systemic total bile acid concentration were determined.
Results: The regeneration rate and the portal pressure were significantly higher in the ALPPS group compared to the PVL group 48h after operation (217.7±12.9 vs. 155±12.1%;p<0,001; 21.3±2.4 vs. 17.5±0.6 mmHg;p=0.023). The transcription of FXR in the liver was significantly decreased in the ALPPS group compare to the PVL group (24h: 0,2±0,1 vs. 0,8±0,2 fold expression;p=0,0321). The portal and systemic bile acid concentration were significantly increased in the ALPPS group (48h:358.0±18.2 vs. 204.5±37,2 µmol/ml;p<0.001, 367.4±82.1 vs. 233,1± 67.9 µmol/ml;p<0.001). The FGFR4 expression of the liver was also significantly higher in the ALPPS group (24h:3,1 ± 0,8 vs. 2,1±0,5 fold expression; p=0.032).
Conclusion: The alterations of the gut-liver axis induced by ALPPS may play an important role in accelerated liver hypertrophy.

Kind

Szabad

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

1025

Authors (legacy)

D.K. Tihanyi1, A. Budai1, T. Kovacs1, A. Fulop1, T.M. van Gulik2, P. Olthof2, A. Szijarto1
1 Semmelweis University, 1st Deparment of Surgery, Budapest, Hungary
2 Academisch Medisch Centrum, Department of Experimental Surgery; Amsterdam, Netherlands