PhD Scientific Days 2017

Oral Presentations: Head&Neck & Basic Sciences I.

Role of microRNA-223 in the activation of poly(adp-ribose) polymerase in pediatric patients with Crohn’s disease

Előadó neve

Béres, Nóra Judit

Előadó munkahelye

Semmelweis University, 1st Department of Pediatrics

Előadó telefonszáma

0630/216-0229

Előadó e-mail címe

beres.nora@med.semmelweis-univ.hu

Az előadás címe

Role of microRNA-223 in the activation of poly(adp-ribose) polymerase in pediatric patients with Crohn’s disease

Szerző(k) neve és munkahelye

Nóra Judit Béres1, Zoltán Kiss1, Rita Benkő2, Katalin Borka3, Apor Veres-Székely1, 4, Szabolcs Heininger5, Rita Lippai1, Ádám Vannay1, 4, Erna Sziksz1, 4, Gábor Veres1, Eszter M. Horváth2

1: Semmelweis University, 1st Department of Pediatrics, Budapest
2: Semmelweis University, Department of Physiology, Budapest
3: Semmelweis University, 2nd Department of Pathology, Budapest
4: MTA-SE, Pediatrics and Nephrology Research Group, Budapest
5: Semmelweis University, Institute of Human Physiology and Clinical Experimental Research, Budapest

Szekció

Oral Presentations: Head&Neck & Basic Sciences I.

Data of the presenter

Doctoral School: Clinical Medicine
Program: Prevention of Crohnic Diseases in Childhood
Supervisor: Gábor Veres
E-mail address: veres.gabor@med.semmelweis-univ.hu

Text of the abstract

Background: Crohn’s disease (CD) is a multifactorial disease, characterized by oxidant-induced tissue injury with a possible activation of the poly(ADP-ribose) polymerase (PARP-1). However, there are no studies examining PARP activation in patients suffering from CD. MicroRNAs (miRs) can offer a potential missing link between the genetic susceptibility, environmental and immunologic factors involved in the pathogenesis of CD. Previously PARP-1 was identified as a direct target gene of miR-223 in an epithelial cell line.
Aims: to examine the level of PARP activation and the expression of miR-223 in colonic biopsies of pediatric CD; to study the role of inflammatory processes, the effect of lipopolysaccharide (LPS) on PARP activation and miR-223 expression is examined in HT-29 colonic epithelial cell line.
Methods: Colonic biopsies were taken from patients with macroscopically inflamed and intact mucosa with CD and controls. LPS treated HT-29 cells serve as our in vitro model. To analyze PARP activation; western blot analysis (antibody against the enzyme and the end product of PARP activation: poly(ADP-ribose) (PAR)), immunohistochemical (anti-PAR) and immunofluorescent (anti-PARP) labeling and real-time PCR (PARP-1 mRNA) were used. To analyze the expression of miR-223 real-time PCR was used.
Results: PARP-1 and miR-223 expression was elevated, however the amount of PARP and PAR was reduced in pediatric CD compared to the controls. The LPS incubation did not affect the expression of PARP-1 mRNA, however decreased the expression of miR-223, and enhanced PARP activation.
Discussion: In our study we showed that the expression of miR-223 is up-regulated and PARP activation is reduced in pediatric patients with CD. Moreover, we confirmed the opposite change in vitro, too. These data suggest that the hypofunctionality of PARP may play a potential role in the pathomechanism of CD.

„SUPPORTED BY THE ÚNKP-16-3-III NEW NATIONAL EXCELLENCE PROGRAM OF THE MINISTRY OF HUMAN CAPACITIES”

Kind

Szabad

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

1045

Authors (legacy)

Nóra Judit Béres1, Zoltán Kiss1, Rita Benkő2, Katalin Borka3, Apor Veres-Székely1, 4, Szabolcs Heininger5, Rita Lippai1, Ádám Vannay1, 4, Erna Sziksz1, 4, Gábor Veres1, Eszter M. Horváth2

1: Semmelweis University, 1st Department of Pediatrics, Budapest
2: Semmelweis University, Department of Physiology, Budapest
3: Semmelweis University, 2nd Department of Pathology, Budapest
4: MTA-SE, Pediatrics and Nephrology Research Group, Budapest
5: Semmelweis University, Institute of Human Physiology and Clinical Experimental Research, Budapest