PhD Scientific Days 2017

Oral Presentations: Cardiovascular

Characterization of a novel mouse model of hepatic cardiomyopathy

Előadó neve

Németh, Balázs

Előadó munkahelye

Heart and Vascular Center, Semmelweis University

Előadó telefonszáma

+3620/663-2946

Előadó e-mail címe

nemethbl@gmail.com

Az előadás címe

Characterization of a novel mouse model of hepatic cardiomyopathy

Szerző(k) neve és munkahelye

Németh BT1,2, Mátyás C1,2, Varga ZV1, Radovits T2, Merkely B2, Pacher P1
1 National Institute on Alcohol Abuse and Alcoholism, Rockville, MD, USA
2 Heart and Vascular Center, Semmelweis University, Budapest, Hungary

Szekció

Oral Presentations: Cardiovascular

Data of the presenter

Doctoral School of Basic Medicine
Program: Cardiovascular Disorders: Physiology and Medicine of Ischaemic Circulatory Diseases
Supervisor: Tamás Radovits
Contact: nemethbl@gmail.com

Text of the abstract

Introduction – Hepatic failure/cirrhosis induces distinct cardiovascular changes described as cirrhotic/hepatic cardiomyopathy. Accurate quantification of this phenomenon, however, is problematic to achieve due to the complex changes affecting both the heart and the vasculature of the animals. We aimed in our current experiments at accurately quantifying functional and structural cardiac changes in mice subjected to 14 days of bile duct ligation (BDL).
Methods – The common bile duct was ligated and severed in young adult mice (n=8). Sham operated animals (n=8) served as controls. At the end of the 14-day observational period, echocardiographic and pressure volume (PV) measurements were carried out in all animals. In addition to our functional evaluation, heart samples were snap-frozen in liquid nitrogen or were fixed in 10% paraformaldehyde to investigate microscopic changes as well.
Results – Our echocardiographic measurements showed a significant decrease in systolic and diastolic left ventricular internal diameter (LVIDs and LVIDd, respectively), stroke volume (SV), cardiac output (CO) and estimated left ventricular mass (LVM) (LVIDd: 3.70±0.04mm vs. 2.64±0.18mm, LVIDs: 2.45±0.08mm vs. 1.30±0.23mm, SV: 28.89±1.32microL vs. 13.40±0.38microL, CO: 15.10±1.12mL vs. 6.18±0.31mL, LVM: 107±4mg vs. 65±11mg, p<0.05), while wall dimensions did not change and systolic functional parameters such as ejection fraction (EF) and fractional shortening (FS), were found to be paradoxically increased (EF: 57±2% vs. 74±6%, FS: 34±2% vs. 52±5%, p<0.05) in our BDL mice compared to control. In contrast, the more sensitive PV measurements revealed both systolic and diastolic dysfunction accompanied by systemic hypotension in BDL mice. Histologic examination showed atrophy of the individual cardiomyocyte and significantly increased collagen area following 14 days of BDL.
Conclusion – Hepatic cardiomyopathy is characterized by systemic hypotension accompanied by both systolic and diastolic dysfunction. The observed cardiomyocyte atrophy and increased left ventricular fibrosis corroborate our functional findings.

Kind

Szabad

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

1135

Authors (legacy)

Németh BT1,2, Mátyás C1,2, Varga ZV1, Radovits T2, Merkely B2, Pacher P1
1 National Institute on Alcohol Abuse and Alcoholism, Rockville, MD, USA
2 Heart and Vascular Center, Semmelweis University, Budapest, Hungary