PhD Scientific Days 2017

Oral Presentations: Oncology

Platelet count, ADAMTS13 activity, von-Willebrand Factor level and survival in patients with colorectal cancer: 5-year follow up study

Előadó neve

Garam, Nóra

Előadó munkahelye

Semmelweis University, 3rd Department of Internal Medicine

Előadó telefonszáma

+3670/6204542

Előadó e-mail címe

norigaram@gmail.com

Az előadás címe

Platelet count, ADAMTS13 activity, von-Willebrand Factor level and survival in patients with colorectal cancer: 5-year follow up study

Szerző(k) neve és munkahelye

Nóra Garam1, Éva Maláti1, Sinkovits György1, Tímea Gombos1, Lóránd Barabás2, László Gráf1, Judit Kocsis1, Zoltán Prohászka1
1 3rd Department of Internal Medicine, Faculty of Medicine, Semmelweis University, Budapest, Hungary
2 2nd Department of Surgery, Faculty of Medicine, Semmelweis University, Budapest, Hungary

Szekció

Oral Presentations: Oncology

Data of the presenter

Doctoral School: Basic Medicine
Program: Clinical and Experimental Cardiology / Atherosclerosis
Supervisor: Dorottya Csuka
E-mail address: norigaram@gmail.com

Text of the abstract

Introduction:
Distant metastasis is a major cause of colorectal cancer related death. There is a growing evidence of an interaction between tumour cells and platelets which promote tumour progression and metastasis formation. This could be mediated by multiple factors such as von Willebrand factor. The most active form of the VWF is the ultralarge VWF which is cleaved by ADAMTS13 protease in physiological circumstances to the multimer form.
Aim:
Our aim was to measure the platelet count, VWF:Ag level and ADAMTS13 activity in a large (n=232) cohort of colorectal cancer patients and to examine their relationships with the presence of metastatic disease and five-year survival using multivariable models.
Method:
ADAMTS13 enzyme activity was determined in citrated plasma samples with the fluorigenic substrate FRETS-VWF73. VWF:Ag level was measured in EDTA plasma by ELISA. Determination of the laboratory by Roche Integra 800 analyzer, by Cell-Dyn 3500 hematology analyzer.
Results:
Significantly higher platelet counts (p=0.005), VWF:Ag levels (p=0.008) and decreased ADAMTS13 activity (p=0.006) was observed in patients with metastatic disease. Results of the Kaplan-Meier analysis showed that lower platelet counts (p<0.0001) and VWF:Ag (p=0.0022) levels, whereas higher ADAMTS13 activity (p<0.0001) was associated with better survival. Finally, to investigate the association between overall survival and the three study variables multivariate Cox proportional hazard models were generated. All models were adjusted for age, gender and disease stage. Platelet count, ADAMTS13 activity or VWF:Ag level were incorporated and all of these variables turned out to be age-, gender- and stage-independent predictors of mortality (all HR >1.6, p<0.05).
Conclusion:
We showed that thrombocytosis, elevated VWF:Ag level and decreased ADAMTS13 activity are independently associate with poor survival in colorectal cancer patients. Therefore, these epidemiological data may support prior observations obtained in animal clinical studies on the contribution of tumour cell-platelet heteroaggregates, hold together by VWF-fibers, in the propagation of tumour progression.

Kind

Szabad

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

1153

Authors (legacy)

Nóra Garam1, Éva Maláti1, Sinkovits György1, Tímea Gombos1, Lóránd Barabás2, László Gráf1, Judit Kocsis1, Zoltán Prohászka1
1 3rd Department of Internal Medicine, Faculty of Medicine, Semmelweis University, Budapest, Hungary
2 2nd Department of Surgery, Faculty of Medicine, Semmelweis University, Budapest, Hungary