PhD Scientific Days 2017

Oral Presentations: Clinical Sciences

Interferon-gamma production-based virus specific T cell detection by functional flow cytometry after allogeneic haematopoietic stem cell transplantation

Előadó neve

Tasnády, Szabolcs

Előadó munkahelye

United St. Istvan and St. Laszlo Hospital, Department of Haematology and Stem Cell Transplantation, Budapest

Előadó telefonszáma

+36704536466

Előadó e-mail címe

sz.tasnady@gmail.com

Az előadás címe

Interferon-gamma production-based virus specific T cell detection by functional flow cytometry after allogeneic haematopoietic stem cell transplantation

Szerző(k) neve és munkahelye

Szabolcs Tasnády 1, Attila Szederjesi 1, Éva Karászi 1, György Bihari 1, Apor Hardi 1, Gergely Kriván 2, Krisztián Kállay 2, Péter Reményi 1, János Sinkó 1, Gábor Mikala 1, Tamás Masszi 3, Marienn Réti 1
1 United St. Istvan and St. Laszlo Hospital, Department of Haematology and Stem Cell Transplantation, Budapest
2 United St. Istvan and St. Laszlo Hospital, Department of Paediatric Haematology and Stem Cell Transplantation, Budapest
3 Semmelweis University III. Department of Internal Medicine, Budapest

Szekció

Oral Presentations: Clinical Sciences

Data of the presenter

Doctoral School: Clinical Medicine
Program: Clinical Haematology
Supervisor: Tamás Masszi
E-mail Address: sz.tasnady@gmail.com

Text of the abstract

Introduction
Morbidity and mortality caused by cytomegalovirus (CMV), adenovirus (ADV) or Epstein-Barr virus (EBV) reactivation remains significant in the allogeneic haematopoietic stem cell transplantation (HSCT) setting. Besides antiviral therapy adoptive transfer of virus specific T cells is an increasingly available and rapid treatment option for these patients.
Aims and methods
Since May 2015, 15 patients have been treated with virus specific T-lymphocyte products from third party donors at our institution. For donor selection a screening procedure of functional flow cytometry based on interferon-gamma (IFNγ) production of T cells was used. Following magnetic selection, graft purity and T cell count was also determined by a similar flow cytometry based technique. To evaluate the clinical efficacy, viral copy numbers measured by PCR methodology were monitored after T cell therapy.
Results
Findings of 168 healthy donors and 251 screening tests were evaluated regarding the frequency of virus specific T cells.
The mean number of IFNγ producing T cells in the graft was highly sufficient for immunotherapy. Viral copy numbers decreased in most cases few weeks after T cell therapy with the resolution of clinical symptoms. No flare of GvHD has been observed. Our preliminary data suggests that there is correlation between the percentage of the IFNγ producing T cells in the blood and the number of T cells in the graft used for immunotherapy.
Conclusion
Administration of virus specific T cells from third party donors selected by CCS technology is an effective antiviral treatment option after allogeneic HSCT. Functional flow cytometry test is a quick and sensitive method for donor screening and for purity check of the T cell grafts, as well as for monitoring immunoreconstitution of T cells after adoptive T cell therapy.

Kind

Szabad

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

1157

Authors (legacy)

Szabolcs Tasnády 1, Attila Szederjesi 1, Éva Karászi 1, György Bihari 1, Apor Hardi 1, Gergely Kriván 2, Krisztián Kállay 2, Péter Reményi 1, János Sinkó 1, Gábor Mikala 1, Tamás Masszi 3, Marienn Réti 1
1 United St. Istvan and St. Laszlo Hospital, Department of Haematology and Stem Cell Transplantation, Budapest
2 United St. Istvan and St. Laszlo Hospital, Department of Paediatric Haematology and Stem Cell Transplantation, Budapest
3 Semmelweis University III. Department of Internal Medicine, Budapest