PhD Scientific Days

Poster Presentation: Oncology

P36: Usefulness of radiosensitizing substance (pentoxifylline) for the treatment of cancer using NKT cells using a solid tumor model in mice

Előadó neve

Lizzeth Madera Sandoval, Ruth

Előadó munkahelye

NATIONAL POLYTECHNIC INSTITUTE

Előadó telefonszáma

+36202419669

Előadó e-mail címe

ruthmaderas@hotmail.com

Az előadás címe

Usefulness of radiosensitizing substance (pentoxifylline) for the treatment of cancer using NKT cells using a solid tumor model in mice

Szerző(k) neve és munkahelye

Madera Sandoval Ruth Lizzeth1, József Tóvrári2, Jiménez Orosco Alejandro3, Vega López Armando1
1 National School of Biologics Sciences, National Polytechnic Institute, Ciudad de México, México
2 Department of Experimental Pharmacology, National Institute of Oncology, Budapest, Hungary
3 Department of Pharmacology, National Autonomous University of México, Ciudad de México, México

Szekció

Poster Presentation: Oncology

Data of the presenter

NATIONAL POLYTECHNIC INSTITUTE
NATIONAL SCHOOL OF BIOLOGICAL SCIENCES
DOCTORATE IN CHEMICAL BIOLOGY
ARMANDO VEGA
József Tóvrári
Jiménez Orosco Alejandro

Text of the abstract

Some molecules are potentially important on tumor cell radio sensitivity while the combination of immunotherapy and these sensitizers could increase the therapeutic potential, there are no studies that analyze possible synergies between these. The pentoxifylline (PTF) a methylxanthine derivative). It has been showed that 0.5 to 1.0 mMOL of PTF elicited an increase in radiation-induced cytotoxicity, whereas the combination of this substance with radiation allowed an increase of the radiosensitization; the earlier studies showed that some alternatives for improvement the activation of NKT cells and survival of specimens with solid tumors exposed to radiotherapy is real. The alpha-galactosylceramide (KRN7000) is a glycosphingolipid able to inhibit tumor growth and to prolong the survival in treated mice. Study aim: To test the therapeutic potential of pentoxifylline in combination with alpha-galactosylceramide treatment and radiotherapy using a solid tumor model in mice. Method: The mice of each group (1:KRN7000, 2: PTF, 3: KRN7000+PTF, 4: control) was inoculated s. c. with 2x105 B16 cells and the tumor size was determinate, we take samples of blood for flow cytometer analysis and the tumor was recollected for immunohistochemistry analysis, the same groups was used for radiotherapy assay . Results: Activation of NKT cells was effective for tumor size reduction in combination with PTF, we can observed more antitumor effect than alone, we observed the survival of the mice was higher in the group with both compound and adding radiotherapy, the number of iNKT was higher in KRN7000 group than the others, and in morphological descriptions we observed positives changes in the KRN7000+PTF+Radiotherapy in comparison with the control groups. Conclusions: the results suggest the use of immunological therapy together with the administration of a radiosensitizing compound plus the main treatment shows morphological improvements in tumor tissue and reduces tumor size.

Azonosító

P36

Kind

Szabad

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

1187

PhD Scientific Days 2017

Budapest, 11-12 April 2017