PhD Scientific Days 2017

Poster Presentation: Clinical Sciences

P25: Setting up a mouse model for repeated moderate noise induced hearing loss

Előadó neve

Szepesy, Judit

Előadó munkahelye

First: Semmelweis University Otorhinolaryngology, Head and Neck Surgery ; Second: Semmelweis University Department of Pharmacology and Pharmacotherapy

Előadó telefonszáma

+36209331187

Előadó e-mail címe

szepesyjudit88@gmail.com

Az előadás címe

Setting up a mouse model for repeated moderate noise induced hearing loss

Szerző(k) neve és munkahelye

Judit Szepesy 1,2, Viktoria Humli 3,2, Laszlo Tamas 1, Anita Gaborjan 1, Tibor Zelles 2,3
1 Semmelweis University Otorhinolaryngology, Head and Neck Surgery, Budapest
2 Semmelweis University Department of Pharmacology and Pharmacotherapy, Budapest
3 Institute of Experimental Medicine of the Hungarian Academy of Sciences, Budapest

Szekció

Poster Presentation: Clinical Sciences

Data of the presenter

Doctoral School: Clinical Medicine
Title of the program: Otorhinolaryngology
Name of the Supervisor: Tibor Zelles, Anita Gaborjan
E-mail address of the presenter: szepesyjudit88@gmail.com

Text of the abstract

Noise induced hearing loss (NIHL) is one of the most common form of sensorineural hearing losses (SNHLs). The prevalence of the disease is increasing by changes in our music listening habits. Despite of their high social relevance, SNHLs have no efficient drug therapy. Drug development requires appropriate animal models. The aim of my study was to set up a NIHL mouse model with high translational value.
The hearing was tested by measuring its threshold by auditory brainstem response (ABR). This method is suitable for several remeasurements. Noise was delivered in a ventilated noise box.
By testing the effect of noise with different intensity and duration in two different mouse strains, first we have deployed a one-time noise exposure mouse model (BALB/c mice; 98 dB, 8 – 16 kHz white noise for 30 min). This noise produced PTS, which was partially attenuated by the known protective substance n-acetyl cysteine and by our candidate compound.
In the next step we started to develop the model of NIHL caused by repeated moderate noise exposure. In everyday life we are regularly exposed to moderate single noise, that cause only temporary threshold shift (TTS), but may easily lead to PTS after repetition. Musical events for children is an example for this situation, as it was explored by the ENT Department of Semmelweis University. We exposed BALB/c mice to 4-18 kHz single white noise at 90, 95, 98, 101 or 105 dB for 15 and 30 minutes. Among these, the 95 dB 15’ noise elicited TTS, but not PTS. Thereafter, mice were repeatedly exposed to this noise four times, once a week and PTS was developed. We are going to repeat the experiments and use our candidate compound to prove the reproducibility and validity of the modell, which will be used for testing potential otoprotective compounds.

Azonosító

P25

Kind

Szabad

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

1205

Authors (legacy)

Judit Szepesy 1,2, Viktoria Humli 3,2, Laszlo Tamas 1, Anita Gaborjan 1, Tibor Zelles 2,3
1 Semmelweis University Otorhinolaryngology, Head and Neck Surgery, Budapest
2 Semmelweis University Department of Pharmacology and Pharmacotherapy, Budapest
3 Institute of Experimental Medicine of the Hungarian Academy of Sciences, Budapest