PhD Scientific Days

Poster Presentation: Neurosciences

P50: Shaping of thalamic network activity by layer 6 corticothalamic feedback

Előadó neve

Balogh, Veronika

Előadó munkahelye

Hungarian Academy of Sciences, Research Centre for Natural Sciences, Budapest

Előadó telefonszáma

+36 1 382 6685

Előadó e-mail címe

balogh.veronika@ttk.mta.hu

Az előadás címe

Shaping of thalamic network activity by layer 6 corticothalamic feedback

Szerző(k) neve és munkahelye

Veronika Balogh, Sándor Borbély, Péter Barthó
NAP-B Sleep Oscillations Research Group, Hungarian Academy of Sciences, Research Centre for Natural Sciences, Budapest

Szekció

Poster Presentation: Neurosciences

Data of the presenter

Doctoral School: János Szentágothai Doctoral School of Neurosciences
Program: Functional Neurosciences
Supervisor: Péter Barthó
Email address of the presenter: balogh.veronika@ttk.mta.hu

Text of the abstract

The thalamus plays a central role in relaying information from the periphery, providing strong feedforward projection to the neocortex. Nevertheless, it acts not just as a simple relay station, but has a more complex role in sensation that is due to the robust feedback projection originating from layer 6 (L6) of the neocortex. This projection outnumbers thalamocortical (TC) projections by a magnitude and may play various roles in the TC network: sharpening the receptive fields of TC cells; influencing the generation of sleep rhythms; acting as classical modulatory pathway in selective attention, yet its actual effect has not been investigated in vivo.
In our research, we use electrophysiological and optogenetic methods to selectively manipulate L6 corticothalamic (CT) neurons in vivo, while simultaneously recording thalamic and cortical activity. We use NTSR1-ChR transgenic mouse line in which L6 CT neurons are exclusively expressing the photosensitive channel-rhodopsin.
Firstly, we investigate the spontaneous firing behaviour of L6 CT neurons under urethane anaesthesia with juxtacellular recordings. The spontaneous activity correlated more with the cortical UP-states, than with the sleep spindles.
Secondly, we investigate the effect of L6 feedback on single thalamic cells and also on thalamic network oscillations. L6 directly excites TC neurons, while it gives collaterals to the nucleus reticularis thalami (nRT), so it also indirectly inhibits them. The net effect of this excitation/inhibition pattern is not clear and may depend on several factors. We show that the projection provides different activation patterns to TC and nRT neurons and the net effect depends on network state and the strength of L6 activation.
Finally, we show that pulse-like activation of L6 can induce sleep spindles depending on the network state both in natural sleep and urethane anaesthesia. Our further goal is to elucidate the spontaneous firing of L6 CT cells in natural sleep.

Azonosító

P50

Kind

Szabad

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

1213

Authors (legacy)