PhD Scientific Days 2017

Oral Presentations: Clinical Sciences

Effects of novel synthetic polypeptides containing ‘cycloRGD’ sequences - on PDLSC functional characterization

Előadó neve

Khorolsuren, Zambaga

Előadó munkahelye

Department of Genetics-, Cell- and Immunobiology, Semmelweis University, Budapest

Előadó telefonszáma

+36307363313

Előadó e-mail címe

zagi1982@gmail.com

Az előadás címe

Effects of novel synthetic polypeptides containing ‘cycloRGD’ sequences - on PDLSC functional characterization

Szerző(k) neve és munkahelye

Zambaga Khorolsuren1, 2, Orsolya Lang 1, Eva Pallinger1, Anna Foldes 3, Kata Horváti4, Gabor Mezo4, Janos Vag 2, Laszlo Kohidai 1
1 Department of Genetics-, Cell- and Immunobiology, Semmelweis University, Budapest
2 Department of Conservative Dentistry, Semmelweis University, Budapest
3 Department of Oral Biology, Semmelweis University, Budapest
4 MTA-ELTE Research Group of Peptide Chemistry, Budapest

Szekció

Oral Presentations: Clinical Sciences

Data of the presenter

Doctoral School: Clinical Medicine
Program: Dental Research
Supervisors: Kőhidai László, Vág János
E-mail: zagi1982@gmail.com

Text of the abstract

Introduction: Survival of the adherent cells is dependent on cell-extracellular matrix (ECM) interactions. Adequate selection of dental materials supports the physiological cell-ECM interaction based wound healing and prevents the nucleation process in and around the dental matrix. Multipotent periodontal ligament stem cells (PDLSCs) are potential targets of dental interventions (periodontitis, tissue engineering). Synthetic biomimetic conjugates composed by substrate-coating molecules (e.g. polylysine) with adhesive peptide motifs are prospective candidates. In our work ’cycloRGD’ motive expressing peptides are investigated.
Objectives: (i) To analyze adhesive and proliferative ability of 3 synthetic polypeptides on PDLSCs. (ii) To analyze effect of peptides on cell migration. (iii) To identify PDLSCs surface molecular characteristics on peptide coated surface. (iv) To analyze effects of synthetic peptides on osteogenic differentiation of PDLSCs.
Materials and methods: Our conjugates are composed by polylysine backbone (SAK, AK) and adhesive peptide side chain cRGDfC or osteopontin derivative. PDLSCs were isolated from human extracted third molars. Techniques applied: (i) Real time impedance assays (xCELLigence) monitored cell adhesion and proliferation. (ii) ibidi® Culture-inserts are used for cell migration assay. (iii) CD markers detected by flowcytometry. (iv) PDLSCs maintained on peptide coated surface with osteogenic induction medium (OM) or regular medium. Real-Time qPCR was used to analyze osteogenic genes expression. ALP and Alizarin Red S staining were performed.
Results: (i) SAK-c[RGDfC] increased PDLSC adhesion in FBS-free culture. (ii) SAK-c[RGDfC] and AK-c[RGDfC] peptides enhanced cell migration and SAK-osteopontin inhibited the migration. (iii) In 1 week, coated peptides decreased exression of cell surface integrins (e.g. CD29, CD49d), while increased MSC markers (e.g. CD105, CD146). (iv) Synthetic peptides enhanced ALP activity and calcium deposits. In the OIM, SAK-c[RGDfC] and AK-c[RGDfC] upregulated RUNX, SAK-osteopontin upregulated ALPL. Without OM, synthetic peptides upregulated bone sialopontin (IBSP).
Conclusion: Our new peptides proved to enhance cell adhesion, migration and support osteogenic differentiation on PDLSCs.

Kind

Szabad

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

1219

Authors (legacy)

Zambaga Khorolsuren1, 2, Orsolya Lang 1, Eva Pallinger1, Anna Foldes 3, Kata Horváti4, Gabor Mezo4, Janos Vag 2, Laszlo Kohidai 1
1 Department of Genetics-, Cell- and Immunobiology, Semmelweis University, Budapest
2 Department of Conservative Dentistry, Semmelweis University, Budapest
3 Department of Oral Biology, Semmelweis University, Budapest
4 MTA-ELTE Research Group of Peptide Chemistry, Budapest