PhD Scientific Days 2017

Oral Presentations: Head&Neck & Basic Sciences I.

Changes of the colon muscular layer and the enteric nervous system in the animal model of acute ulcerative colitis

Előadó neve

Khochanskiy, Dmitry, PhD

Előadó munkahelye

Research Institute of Human Morphology, Moscow, Russia

Előadó telefonszáma

89165430584

Előadó e-mail címe

demetros87@gmail.com

Az előadás címe

Changes of the colon muscular layer and the enteric nervous system in the animal model of acute ulcerative colitis

Szerző(k) neve és munkahelye

Khochanskiy D.N. 1, Makarova O.V. 1, Buravkov S.V. 2, Kirukhin S.O.1 2
1 Research Institute of Human Morphology, Moscow, Russia;
2 Lomonosov Moscow State University, Moscow, Russia

Szekció

Oral Presentations: Head&Neck & Basic Sciences I.

Data of the presenter

Doctoral school: Basic Medicine
Program: Cell biology and histology
Supervisor: Makarova O.V.
E-mail: makarov.olga2013@yandex.ru

Text of the abstract

Introduction: One of the main clinical manifestation of inflammatory bowel diseases such as ulcerative colitis or Crohn’s is the alteration of the colon motoric activity, which depends on the enteric nervous system and muscular coat. Morphological changes in the colon muscular coat and enteric nervous system are described both in humans and animal models, however there is a demand for their complex evaluation.
Aims: to describe and quantify the changes in the distal colon enteric nervous system and muscular coat using a murine model of acute ulcerative colitis.
Methods: Adult male C57Bl/6 mice were used. Acute colitis was induced by 2.5-5% aquatic solution of dextran sodium sulfate salt. The distal colon was utilized. Hematoxylin and Nissl staining was used on histological slices and immunolabeling was used on acetone and PFA fixed whole mounts with the anti-βIII-tubulin (Abcam), anti-HuC/D (Thermofisher) antibodies and DAPI. Digital images were obtained with Leica DM2000, Zeiss Axioplan 2 and LSM 780 and analyzed with ImageJ.
Results: The dextran sodium sulfate salt consumption caused acute colitis with a 1.5-fold decrease in the total colon length. The normal mucosa was replaced with inflammatory granulation-like tissue. There was a massive inflammatory infiltration and edema of the submucosal layer. Despite this the muscular layer was relatively intact with little inflammation and there was no increase in the muscular layer thickness on both histological slices and whole mounts. Nevertheless, the myenteric ganglia density per length had a 3-fold increase, as well as the number of intramuscular nerve fibers, which increased by 25% and mucosal nerve fibers area, which had a 7.1-fold increase.
Conclusion: Despite the colon shortening there was no increase in the muscular coat thickness, yet there was an increase in number of myenteric ganglia, intramuscular and mucosal fibers, which may reflect the plasticity of these components of the colon.

Kind

Szabad

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

1229

Authors (legacy)

Khochanskiy D.N. 1, Makarova O.V. 1, Buravkov S.V. 2, Kirukhin S.O.1 2
1 Research Institute of Human Morphology, Moscow, Russia;
2 Lomonosov Moscow State University, Moscow, Russia