PhD Scientific Days

Poster Presentation: Neurosciences

P56: Study on D-serine transport into SH-SY5Y neuroblastoma cells by chiral capillary electrophoresis

Előadó neve

Vincze, István

Előadó munkahelye

Department of Pharmacodynamics

Előadó telefonszáma

+36703872449

Előadó e-mail címe

vincze.istvan@pharma.semmelweis-univ.hu

Az előadás címe

Study on D-serine transport into SH-SY5Y neuroblastoma cells by chiral capillary electrophoresis

Szerző(k) neve és munkahelye

István Vincze1, Tamás Tábi1, Éva Szökő1
1 Semmelweis University, Department of Pharmacodynamics, Budapest, Hungary

Szekció

Poster Presentation: Neurosciences

Data of the presenter

Doctoral: School: Pharmaceutical Sciences
Program: Experimental and Clinical Pharmacology
Éva Szökő
szoko.eva@pharma.semmelweis-univ.hu

Text of the abstract

Introduction: Hypofunction of N-methyl D-aspartate (NMDA) type glutamate receptor has been associated with numbers of central nervous system diseases, including schizophrenia and bipolar disorder. D-serine is a co-agonist of NMDA receptor thus decreased level of this activator/neuromodulator compound may play a key role in the pathophysiological mechanisms. The extracellular D-serine concentration is supposed to be regulated by different transport systems in the CNS.

Aim: To examine and characterize the D-serine uptake in SH-SY5Y neuroblastoma cell line.

Methods: SH-SY5Y cells were incubated with different concentrations of D-serine, which was followed by the determination of the intracellular D-serine content by a capillary electrophoresis with laser induced fluorescence (CE-LIF) detection method, which was previously developed in our laboratory. Sodium dependency of the uptake mechanisms was also tested. The effect of various transport inhibitors on the intracellular D-serine level was examined, as well.

Results: D-serine uptake in SH-SY5Y cell line showed a time- and dose dependent characteristics. The steady state of the transport process was reached after 4 hours, though even after 2 hours the intracellular concentration was over 90% of the steady state value. After saturation the intracellular D-serine concentration was 6-8 times higher than the concentration of the incubating solutions suggesting its active accumulation. More than 89% of the transport was found to be sodium-dependent. Both L-glutamine and trans-4-Hydroxy-L-proline inhibited the transport dose-dependently; however L-glutamine was proved to be a more potent inhibitor.

Conclusion: The uptake of D-serine in SH-SY5Y cell line is a time-, and dose-dependent active transport. The process is mostly sodium-dependent and can be potently inhibited by L-glutamine indicating that ASCT2 transporter is supposed to be involved in D-serine uptake into SH-SY5Y cells.

Azonosító

P56

Kind

Szabad

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

1263

Authors (legacy)