PhD Scientific Days 2017

Oral Presentations: Basic Sciences II.

Explorative analysis of the dynamics of the murine CD103+ cytotoxic resident memory T cell responses during influenza reinfection

Előadó neve

Dr. Bencsik, András

Előadó munkahelye

Semmelweis University, Department of Genetics, Cell- and Immunobiology

Előadó telefonszáma

+36 1 210 2930/56107

Előadó e-mail címe

bencsikandras89@gmail.com

Az előadás címe

Explorative analysis of the dynamics of the murine CD103+ cytotoxic resident memory T cell responses during influenza reinfection

Szerző(k) neve és munkahelye

András Bencsik1,2, Barbara Érsek1,2, Nikolett Lupsa1,2, István Jankovics3, János Matkó4, Edit Irén Buzás2, Zoltán Pós1,2
1 Experimental and Translational Immunomics Research Group, Budapest
2 Semmelweis University, Department of Genetics, Cell and Immunobiology, Budapest
3 National Center for Epidemiology, Division of Virology, Budapest
4 Eötvös Loránd University, Department of Immunology, Budapest

Témacsoport

molecular sciences

Szekció

Oral Presentations: Basic Sciences II.

Data of the presenter

Doctoral School: Molecular Medicine
Program: Basis of Human Molecular Genetics and Gene Diagnostics
Supervisor: Zoltán Pós
E-mail address: bencsikandras89@gmail.com

Text of the abstract

CD8+ resident memory T cells (Trm) are sentinel-like memory cells maintaining an early warning system and also constituting the first line of defense against various recurring viral infections in diverse tissues. Nonetheless, the exact contribution of CD8+ Trm cells to recall antiviral immune responses is still elusive, in part because it seems to be fine-tuned by both the viral entity in question, and local tissue environs.

This study aims at a better understanding of the earliest response mechanisms initiated by lung-resident murine CD8+ Trm cells activated by influenza reinfection in vivo.

Lung resident CD8+ Trm cells were generated in naïve C57Bl/6J mice by intranasal infection using sublethal dose (150xTCID50) of mouse-adapted influenza A/PR/8/34 (H1N1), and subsequently activated by lethal dose viral challenge (150.000xTCID50) six weeks later. Successful viral infection was confirmed by monitoring weight loss, performing lung histology, and influenza-specific immunohistochemistry. Next, lungs were subjected to automated tissue dissociation using a GentleMACS Octo platform, and establishment of virus-specific CD8+ resident memory was validated by enumerating nucleoprotein-specific Trm cells with MHC tetramer staining. Finally, live CD8b+CD69+CD103hi (Trm) cells were FACS-sorted from dissociated lungs, harvested on days 0, 1, 2, and 3 after reinfection. Isolated CD8+ Trm cells were analyzed by transcriptome profiling and flow cytometry. CD8+ Trm-specific changes were identified by using pulmonary non-resident CD8+ T cells as reference.

Preliminary findings suggest that influenza-induced expression of several transcription factors, chemokine receptors and metalloproteases is restricted to the CD8+ Trm subset during influenza re-infection.

These data suggest that a systematic comparison of the dynamics of CD8+ Trm and non-Trm responses may disclose previously hidden aspects of the activity of tissue-resident memory cells in recurrent viral infections.

Kind

Szabad

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

1275

Authors (legacy)

András Bencsik1,2, Barbara Érsek1,2, Nikolett Lupsa1,2, István Jankovics3, János Matkó4, Edit Irén Buzás2, Zoltán Pós1,2
1 Experimental and Translational Immunomics Research Group, Budapest
2 Semmelweis University, Department of Genetics, Cell and Immunobiology, Budapest
3 National Center for Epidemiology, Division of Virology, Budapest
4 Eötvös Loránd University, Department of Immunology, Budapest