PhD Scientific Days 2017

Oral Presentations: Pharmaceutical

Isomerization study of the phenylethanoid acteoside isolated from its new and rich source: gall of Fraxinus angustifolia

Előadó neve

Zürn, Moritz

Előadó munkahelye

ELTE TTK, Institute of Biology, Department of Plant Anatomy

Előadó telefonszáma

06 30 844 7764

Előadó e-mail címe

moritz.zuern@gmail.com

Az előadás címe

Isomerization study of the phenylethanoid acteoside isolated from its new and rich source: gall of Fraxinus angustifolia

Szerző(k) neve és munkahelye

Moritz Zürn
Department of Plant Anatomy, Institute of Biology, ELTE TTK, Budapest

Szekció

Oral Presentations: Pharmaceutical

Data of the presenter

Doctoral School: Doctoral School of Pharmaceutical Sciences
Program: Directions of modern research in pharmaceutical sciences
Supervisor: Imre Boldizsár
E-mail address: moritz.zuern@gmail.com

Text of the abstract

Isomerization study of the phenylethanoid acteoside isolated from its new and rich source: gall of Fraxinus angustifolia

Acteoside is a valuable plant secondary metabolite belonging to phenylthanoid glycosides. It is an important anti-microbial, anti-fungal, anti-cancer, anti oxidative and neuroprotective agent, which can mainly be found in the representatives of Lamiales and Asterales plants. Thus, also Fraxinus spp. were found to be acteoside containing. Plant galls are abnormal outgrowths of plant tissues which are caused by the influence of insects. They could be regarded as special tumors of plants. Based on this, our hypothesis was that the high cell division rate in the galls might result in an increased synthesis of secondary metabolites, characteristic to the host plant.
In order to characterize the conversion of acteoside into its closely related compounds, extracts were heated with methanol, water and 2 N trifluoroacetic acid (TFA) at 50 °C and 100 °C, applying different incubation periods.
As a result of our work, an extremely high amount of acteoside was determined in the gall of Fraxinus angustifolia (16,6 mg/g) for the first time, thus confirming the most abundant source of this compound in the plant kingdom. Following the conversion characteristic of acteoside, its three related compounds (i.e. desrhamnosyl acteoside, isoacteoside and desrhamnosyl isoacteoside) were identified by HPLC-UV-MS. The optimum treatment conditions, resulting in the highest levels of the selected products, were determined as follows: TFA, 100 °C, 15 min. Applying these conditions, all conversion products were subsequently isolated by preparative HPLC for further structure identification and pharmacological tests.
In conclusion, in addition to acteoside, ater an optimized acid treatment, three phenylethanoids occurring only in trace amounts in plants could also be isolated from the gall of Fraxinus angustifolia.

Kind

Szabad

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

1279

Authors (legacy)

Moritz Zürn
Department of Plant Anatomy, Institute of Biology, ELTE TTK, Budapest