PhD Scientific Days 2017

Oral Presentations: Basic Sciences II.

Emerging roles of pregnancy-associated circulating extracellular vesicles in modulation of trophoblast cell functions

Előadó neve

Dr. Kovács, Árpád Ferenc

Előadó munkahelye

Semmelweis University, Department of Genetics, Cell and Immunobiology

Előadó telefonszáma

0036307875026

Előadó e-mail címe

dr.arpad@yahoo.com

Az előadás címe

Emerging roles of pregnancy-associated circulating extracellular vesicles in modulation of trophoblast cell functions

Szerző(k) neve és munkahelye

Á. F. Kovács1, N. Fekete1, O. Láng1, L. Kőhidai1, J. Rigó2, L. Turiák3, E. I. Buzás1, É. Pállinger1
1 Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary
2 1st Department of Obstetrics and Gynaecology, Semmelweis University, Budapest, Hungary
3 Institute of Organic Chemistry, Hungarian Academy of Sciences, Budapest, Hungary

Témacsoport

molecular sciences

Szekció

Oral Presentations: Basic Sciences II.

Data of the presenter

Doctoral School of Molecular Medicine
Program: Basis of Human Molecular Genetics and Gene Diagnostics
Supervisor: Éva Pállinger
E-mail: eva.pallinger@gmail.com

Text of the abstract

Introduction: Extracellular vesicles (EV) represent a critically important, directed intercellular communication pathway during pregnancy. Circulating EV patterns are associated with pregnancy complications, and may exert divergent effects on target cells.
Aim: The aim of our study was to identify the effects of circulating pregnancy-associated EVs on trophoblasts, as potential target cells.
Methods: The effects of pregnancy-associated EVs were monitored by the using of BeWo choriocarcinoma cell line as a model system. EV-enriched preparations were isolated from the plasma of healthy (HP-EV) or preeclamptic pregnants (PE-EV) by differential centrifugation and were characterized by flow cytometry, dynamic light scattering, confocal laser scanning microscopy (CLSM), transmission electron microscopy and mass spectrometry. Adhesiveness and migratory activity of EV-treated BeWo cells were assessed by an impedance- based real-time method (xCELLigence SP) and by holographic microscopy (Holo Monitor M4). Trophoblast invasion and placental remodelling was examined by a wound-healing assay.
Results: Pregnancy-associated EVs isolated from each group augmented the adhesion kinetics of BeWo cells, although the effect of PE-EVs was less pronounced. Furthermore, PE-EVs induced reduced cell migration and motility. Conversely, the invasiveness of BeWo cells was intensified in the presence of PE-EVs. The differential effects of circulating EVs isolated from healthy and preeclamptic patients could be explained by their distinct protein cargos as suggested by by mass spectrometry.
Conclusion: Our in vitro experiments suggest that pregnancy-associated circulating EVs affect the invasion of trophoblast cells, which may be very important in the regulation of spiral artery remodelling and ultimately, the placentation.

Kind

Szabad

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

1283

Authors (legacy)

Á. F. Kovács1, N. Fekete1, O. Láng1, L. Kőhidai1, J. Rigó2, L. Turiák3, E. I. Buzás1, É. Pállinger1
1 Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary
2 1st Department of Obstetrics and Gynaecology, Semmelweis University, Budapest, Hungary
3 Institute of Organic Chemistry, Hungarian Academy of Sciences, Budapest, Hungary