PhD Scientific Days 2017

Poster Presentation: Pharmaceutical

P06: Alteration of insulin receptor substrate 1 (IRS-1) phosphorylation in different tissues in diet induced obesity in rats

Előadó neve

Bagaméry, Fruzsina

Előadó munkahelye

Department of Pharmacodynamics, Semmelweis University

Előadó telefonszáma

+36303435219

Előadó e-mail címe

bfruzsina11@gmail.com

Az előadás címe

Alteration of insulin receptor substrate 1 (IRS-1) phosphorylation in different tissues in diet induced obesity in rats

Szerző(k) neve és munkahelye

Fruzsina Bagaméry1, Éva Szökő1, Róbert Gáspár2, Tamás Tábi1
1 Department of Pharmacodynamics, Semmelweis University, Budapest, Hungary
2 Department of Pharmacodynamics and Biopharmacy, University of Szeged, Szeged Hungary

Szekció

Poster Presentation: Pharmaceutical

Data of the presenter

Doctoral School: Pharmaceutical Sciences
Program: Experimental and Clinical Pharmacology
Supervisor: Tamás Tábi
tabi.tamas@pharma.semmelweis-univ.hu

Text of the abstract

Introduction: Recently published papers outlined the gender differences in insulin resistance and suggest a solid association between the metabolic status, insulin signaling and other endocrine mechanism.
Aim: To examine the gender dependent mechanism of tissue insulin resistance in high-fat-high-sugar-diet (HFHSD) induced obesity.
Method: Animal experiments and tissue collection were performed at University of Szeged. Male and female rats were fed either by standard or HFHS diet for 20 weeks. The effect of diet induced obesity on insulin signaling was assessed gender specifically. Plasma insulin level and IRS-1 phosphorylation in tissues were measured by ELISA.
Results: Similar extant of hyperinsulinemia was induced by HFHSD both in female and male animals but considerable interindividual differences were observed in the obese group. The elevated plasma insulin concentration was accompanied with normal fasting plasma glucose levels thus state of compensated insulin resistance can be speculated. Calculated HOMA-IR index also confirmed similar systemic insulin resistance in both genders. Studying the molecular mechanism of insulin resistance on the other hand we found both gender and tissue differences. Significant decrease in tyrosine phosphorylation of IRS-1 was detected only in the liver of obese male rats while female rats showed similar level as seen in the control diet group. In the striated muscle, no HFHSD induced reduction in the IRS-1 tyrosine phosphorylation was observed in any genders.
Conclusion: HFHSD induced mild, compensated insulin resistance mainly deriving from the liver. Male rats were showed more considerable susceptibility for the development of diet induced impaired insulin sensitivity.
Acknowledgement: The project was supported by Bohdan Malaniak Young Scientists Research Grant of the Association for Regional Cooperation in the Fields of Health, Science and Technology (RECOOP HST Association) and Cedars Sinai Medical Center’s International Research and Innovation in Medicine Program.

Azonosító

P06

Kind

Szabad

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

1319

Authors (legacy)

Fruzsina Bagaméry1, Éva Szökő1, Róbert Gáspár2, Tamás Tábi1
1 Department of Pharmacodynamics, Semmelweis University, Budapest, Hungary
2 Department of Pharmacodynamics and Biopharmacy, University of Szeged, Szeged Hungary