PhD Scientific Days 2017

Poster Presentation: Neurosciences

P69: Examination of the septo-hippocampal cholinergic pathway

Előadó neve

Schlingloff, Dániel

Előadó e-mail címe

schlingloff.daniel@koki.mta.hu

Az előadás címe

Examination of the septo-hippocampal cholinergic pathway

Szerző(k) neve és munkahelye

Dániel Schlingloff1, 2, Virág Tresóné-Takács1, Csaba Cserép1, Gábor Nyíri1, Tamás F. Freund1, Attila I. Gulyás1
1 Laboratory of Cerebral Cortex Research, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest
2 János Szentágothai School of Neurosciences, Semmelweis University, Budapest

Szekció

Poster Presentation: Neurosciences

Data of the presenter

D.S. is Supported by the ÚNKP-16-3-IV New National Excellence Program of the Ministry of Human Capacities.
Doctoral School: János Szentágothai School of Neurosciences
Program: Functional Neurosciences
Supervisor: Attila I. Gulyás
E-mail address: schlingloff.daniel@koki.mta.hu

Text of the abstract

The hippocampus is an archicortical structure, which plays important role in the formation of long-term memory traces. Two major physiological activity patterns are present in the hippocampus. Theta modulated gamma oscillations dominate the hippocampal EEG during active exploration, while irregular sharp wave-ripple oscillations appear in rest periods and slow-wave sleep. Subcortical modulatory transmitter systems (e.g. cholinergic, serotonergic, dopaminergic) thought to play important role in setting these distinct activity states. Medial septal cholinergic pathway targeting the hippocampal formation plays key role in the generation of theta oscillations. Recent works suggest that cholinergic neurons of the basal forebrain also contain GABA, the main inhibitory neurotransmitter of the central nervous system. In this work we examined, whether cholinergic neurons of the medial septum shares this phenomenon. To address this question, we injected CRE-dependent adeno-associated viruses carrying channelrhoposin-2 into the medial septum of choline acetyltransferase-CRE mice. This method enables the selective optical excitation of cholinergic fibers targeting the hippocampus. After appropriate expression time, hippocampal slices were prepared for in vitro electrophysiological examination of cellular and network level responses to cholinergic fiber stimulation. Our results reveal the functional GABAergic transmitter phenotype of medial septal cholinergic neurons, where GABA release plays important role in controlling hippocampal network activity patterns.

Azonosító

P69

Kind

Szabad

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

1541

Authors (legacy)

Dániel Schlingloff1, 2, Virág Tresóné-Takács1, Csaba Cserép1, Gábor Nyíri1, Tamás F. Freund1, Attila I. Gulyás1
1 Laboratory of Cerebral Cortex Research, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest
2 János Szentágothai School of Neurosciences, Semmelweis University, Budapest