PhD Scientific Days 2018

Oral presentations: Pathology

ROLE OF NANOVESICLES IN THE DEVELOPMENT OF PERITONEAL FIBROSIS DURING PERITONEAL DIALYSIS

Előadó neve

Dr. Hriczóné Dr. Szebeni, Beáta, PhD

Előadó munkahelye

(1) MTA-SE, Pediatrics and Nephrology Research Group, Budapest, Hungary, (2) Ist Department of Pediatrics, Semmelweis University, Budapest, Hungary

Előadó telefonszáma

+36-20-585-2627

Előadó e-mail címe

szebeni@med.semmelweis-univ.hu

Az előadás címe

ROLE OF NANOVESICLES IN THE DEVELOPMENT OF PERITONEAL FIBROSIS DURING PERITONEAL DIALYSIS

Szerző(k) neve és munkahelye

Beáta Szebeni (1,2), István Márton Takács (2), Domonkos Pap (1,2), Apor Veres-Székely (2), György Reusz (2), Attila J. Szabó (1,2), Ádám Vannay (1,2)
1 MTA-SE, Pediatrics and Nephrology Research Group, Budapest, Hungary
2 Ist Department of Pediatrics, Semmelweis University, Budapest, Hungary

Bemutatás módja

Szóbeli

Szekció

Oral presentations: Pathology

Language of the presentation

Hungarian

Témacsoport

molecular sciences

Összefoglaló szövege

Introduction: Peritoneal fibrosis can be observed almost all patients undergoing peritoneal dialysis (PD), however some patients survive for long periods on PD, others suffer from fibrosis-related membrane failure in short time.
Aims: The present study has been dedicated to search for an explanation of this phenomenon.
Methods: We have enrolled PD effluents from patients (n=7, 5 boys and 2 girls, median age 17.1 [2-31,3] years) receiving continuous ambulatory peritoneal dialysis (CAPD) treatment in the 1st Department of Pediatrics, Semmelweis University. All patients were dialysed with 1.5% glucose containing PD solution at the time of sample collection. Primary peritoneal fibroblasts (pPF) were isolated from PD effluents of these patients. To test the ability of these pPFs to proliferate or produce exracellular matrix (ECM) components MTT/LDH test or Sirius-red staining have been performed, respectively. The ability of cell free PD effluents collected from these PD patients to stimulate proliferation and ECM production of NRK-49F cells were measured by using the same methods.
Results: pPFs were successfully isolated from PD effluents and were identified as fibroblasts based on their morphology, α-smooth muscle actin (SMA) immunopositivity and cytokeratine negative staining. Proliferation rate of these primary peritoneal fibroblasts was increased after PDGF-BB treatment compared to vehicle treated controls. Moreover, these cells also responded to TGF-β treatment with increased collagen production. We observed increased proliferation rate of NRK-49F cells after cell free PD effluent treatment compared to PD solution treated controls. This elevation was highest in case of the patient who underwent PD for 10 months and lowest in case of the patient who underwent PD for 4 years. In paralell, LDH level was highest in case of the patient who underwent PD for 4 years.
Conclusion: Our proposed work may support the early identification of patients receiving PD with high risk of developing peritoneal fibrosis.

Data of the presenter

ÚNKP-17-4-IV-SE-60
email: szebeni@med.semmelweis-univ.hu

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

2915

Authors (legacy)

Beáta Szebeni (1,2), István Márton Takács (2), Domonkos Pap (1,2), Apor Veres-Székely (2), György Reusz (2), Attila J. Szabó (1,2), Ádám Vannay (1,2)
1 MTA-SE, Pediatrics and Nephrology Research Group, Budapest, Hungary
2 Ist Department of Pediatrics, Semmelweis University, Budapest, Hungary