PhD Scientific Days 2018

Oral presentations: Molecular medicine

Benzaldehyde-induced specific adaptive cellular stress and detoxification responses in C. elegans

Előadó neve

Hajdú, Gábor, PhD

Előadó munkahelye

Semmelweis University, Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Budapest, Hungary

Előadó telefonszáma

06707700697

Előadó e-mail címe

hg1313@gmail.com

Az előadás címe

Benzaldehyde-induced specific adaptive cellular stress and detoxification responses in C. elegans

Szerző(k) neve és munkahelye

Hajdú Gábor1, Taisz István1,2
1 Semmelweis University, Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Budapest, Hungary
2 MRC Laboratory of Molecular Biology, Neurobiology Department, Cambridge, UK

Bemutatás módja

Szóbeli

Szekció

Oral presentations: Molecular medicine

Language of the presentation

Hungarian

Témacsoport

molecular sciences

Összefoglaló szövege

Introduction: In response to tissue damaging noxae, conserved cellular stress responses, xenobiotic detoxification and aversive behaviour facilitate organismal survival. The nematode Caenorhabditis elegans exhibits a concentration dependent biphasic behavioural response to the food ingredient volatile benzaldehyde (BA). Concentrated BA evokes an initial attraction followed by a strong aversion. However, whether aversion is a consequence of acute chemosensation or delayed direct tissue damage is unknown.
Aims: In this study we investigate BA-dependent toxicity, the protective cellular responses and their relationship to the aversive behavior.
Methods: We performed hanging-drop odor exposition experiments for investigating BA-caused organismal effects on acute survival and thermotolerance, and fluorescence microscopy measurements for analyzing adaptive cellular responses of animals. At the behavioural level, lawn-avoidance and chemotaxis assays were carried out.
Results: We demonstrated that BA-treatment induces dose dependent paralysis, death, and reduces thermotolerance. At cellular level, exposure to BA specifically triggered nuclear translocation of the stress-responsive DAF-16/FOXO transcription factor and elevated the expression of the phase-I detoxification enzyme cyp-35b::GFP reporter construction. Further, BA robustly increased autofluorescence of the intestinal aging marker Lysosome Related Organelles (LRO). Interestingly, experiments using LRO biogenesis mutants revealed that LRO-granules are required for survival and thermotolerance upon BA exposure. Currently, employing mutants we test how the abovementioned protective cellular responses regulate the BA-induced avoidance and adaptation.
Conclusion: Our work beyond providing the first evidence on the protective role of C. elegans LRO-s may shed light on the molecular mechanisms involved in benzaldehyde induced toxicity and adaptive aversiveness.

Data of the presenter

Doctoral School of Molecular Medicine
Pathobiochemistry
Supervisor: Dr. Csaba Sőti
Email: soti.csaba@med.semmelweis-univ.hu
Oral presentation

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

2767

Authors (legacy)

Hajdú Gábor1, Taisz István1,2
1 Semmelweis University, Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Budapest, Hungary
2 MRC Laboratory of Molecular Biology, Neurobiology Department, Cambridge, UK