PhD Scientific Days 2019

Poster presentations: Neurosciences

Using patterned optogenetic stimulation to reveal integrative properties of hippocampal inhibitory neuron subtypes

Előadó neve

Berki, Péter

Előadó munkahelye

Institute of Experimental Medicine - HAS

Előadó telefonszáma

+36306156522

Előadó e-mail címe

berki.koki@gmail.com

Az előadás címe

Using patterned optogenetic stimulation to reveal integrative properties of hippocampal inhibitory neuron subtypes

Szerző(k) neve és munkahelye

Peter Berki1,2, Andras Vidosits1, Diego Lopez-Pigozzi1, Zsolt Kohus1, Daniel Schlingloff1,2, Tamas F. Freund1 and Attila I. Gulyas1

1: Institute of Experimental Medicine - Hungarian Academy of Sciences, Laboratory of Cerebral Cortex Research; Budapest (HU)
2: Semmelweis University - János Szentágothai Doctoral School, Functional Neurosciences Program; Budapest (HU)

Bemutatás módja

Poszter

Szekció

Poster presentations: Neurosciences

Language of the presentation

English

Témacsoport

neurosciences

Összefoglaló szövege

Introduction: Inhibitory neurons (IN) have a crucial role in shaping network dynamics of the hippocampal formation. INs are diverse in many features, like dendritic geometry, distribution of inputs and variations in ion channels, assigning each type a specific integrative property.
Aims: We wanted to systematically measure the spatial and temporal integrative properties of different IN classes. So far, studies addressing this question activated the same input (i.e.: pathway, subset of axons or synapse) repeatedly. Therefore the possible effects of short-term plasticity contaminated the characterization of temporal properties. Furthermore, spatial and temporal interactions among different inputs is another important element of integration.
Method: Here we present an approach that allows us to stimulate a neuron through temporally and spatially precise combinations of many inputs by using optogenetics combined with a digital micro-mirror device (DMD).
Questions:
• Can we evoke synaptically driven responses in neurons that are mimicking the physiological inputs from the network?
• Can we measure the transmission properties of many input pathways at the same time (rise / decay / amplitude / STP)?
• Can we measure the spatial and temporal interaction of multiple synaptically driven inputs or membrane currents?
• Are there any differences among the integrative properties of different IN subtypes?
Conclusions:
• Independent, synaptically driven responses (indirect stimulation) or membrane currents (direct stimulation) can be persistently and precisely evoked.
• Optically evoked synaptic responses show the probabilistic property of transmission.
• Individual inputs might show distinct STP properties and frequency dependent transmission.
• By examining the interaction of multiple inputs, we concluded that different types can show different integrative properties, e.g. preferred integration time window.

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4078

Presentation file URL

//docs.kmcongress.com/phd2019/eposter_5957.jpeg

Authors (legacy)

Peter Berki1,2, Andras Vidosits1, Diego Lopez-Pigozzi1, Zsolt Kohus1, Daniel Schlingloff1,2, Tamas F. Freund1 and Attila I. Gulyas1

1: Institute of Experimental Medicine - Hungarian Academy of Sciences, Laboratory of Cerebral Cortex Research; Budapest (HU)
2: Semmelweis University - János Szentágothai Doctoral School, Functional Neurosciences Program; Budapest (HU)

Data of the presenter

Doctoral School: Semmelweis University – János Szentágothai Doctoral School
Program: Functional Neurosciences
Supervisor: Attila Gulyas
E-mail address: berki.koki@gmail.com