PhD Scientific Days 2019

Oral presentations: Pharmaceutical sciences

The analgesic effect of sarcosine, a glycine transporter-1 inhibitor in nociceptive and neuropathic pain in rats

Előadó neve

Dr. AMIR, MOHAMMADZADEH

Előadó munkahelye

Department of pharmacology and pharmacotherapy, Semmelweis University.

Előadó telefonszáma

0036706682211

Előadó e-mail címe

amir.ngc11@gmail.com

Az előadás címe

The analgesic effect of sarcosine, a glycine transporter-1 inhibitor in nociceptive and neuropathic pain in rats

Szerző(k) neve és munkahelye

Amir Mohammadzadeh
Department of Pharmacology and Pharmacotherapy, Semmelweis University, Budapest.

Bemutatás módja

Szóbeli

Szekció

Oral presentations: Pharmaceutical sciences

Language of the presentation

English

Témacsoport

pharmaceutical sciences

Összefoglaló szövege

Introduction: Recently growing data support the analgesic action of the glycine transporter inhibitors. However, controversial data have also been reported on their antinociception. Therefore, GlyT-1 inhibitors might be promising analgesic agents, especially for the treatment of neuropathic pain.

Aims: Investigation of the effects of the GlyT-1 inhibitor sarcosine after acute and chronic treatment: 1) acute nociceptive thermal test: tail-flick test; 2) acute inflammatory model: formalin test; 3) mononeuropathic model.

Methods: Male Wistar rats (150-300 g) were used. Tail-flick test: the tail-flick reaction was evoked by a light focused on the tail of rats. The reaction time was expressed in Sec. Measurements were carried out before and after acute-, and chronic (3 and 7 days, twice daily) treatments. Formalin test: 2.5% formalin was injected into the right hind paw and nociceptive reactions were calculated for 60 min. Sarcosine’s effect was assessed following acute or 3 days of treatment. Mononeuropathic model: Right side sciatic nerve was partially ligated (Seltzer ligation). Tactile sensitivity was tested by dynamic plantar aesthesiometer. Sarcosine effect was determined after acute-, 3 and 5 days of treatment. The drug or vehicle was subcutaneous (sc.) injected.

Results: Systemic sarcosine (1000 mg/kg) showed significant antinociception in rat tail-flick assay following 3 and 7 days of treatment. Sarcosine failed to affect pain reactions in Formalin test. Systemic sarcosine (500 mg/kg) showed antiallodynic effect in mononeuropathic pain model after acute, 3 and 5 days of treatment. In addition, no effect was measured in the intact paws.

Conclusion: Acute GlyT-1 inhibition does not alleviate thermal pain, however, chronic GlyT-1 inhibition results in thermal antinociception. In neuropathic model, both acute and chronic treatment successfully alleviated mechanical allodynia. These data suggest that GlyT-1 might be a novel therapeutic target in the treatment of neuropathic pain, which is still a huge clinical challenge.

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4096

Authors (legacy)

Amir Mohammadzadeh
Department of Pharmacology and Pharmacotherapy, Semmelweis University, Budapest.

Data of the presenter

Doctoral School: Doctoral school of pharmaceutical sciences
Program: Experimental and Clinical Pharmacology
Supervisor: Mahmoud Alkhrasani
E-mail address: al-khrasani.mahmoud@med.semmelweis-univ.hu