PhD Scientific Days 2019

Poster presentations: Immunology

ROLE OF CXCR4-CXCL12 SIGNALING IN COLONIZATION OF DEVELOPING BURSA OF FABRICIUS

Előadó neve

Ms. Halasy, Viktória, MSc

Előadó munkahelye

Laboratory of Stem Cells and Experimental Embryology, Department of Anatomy, Histology and Embryology, Faculty of Medicine, Semmelweis University, Budapest, Hungary

Előadó telefonszáma

+36204228797

Előadó e-mail címe

viktoria.halasy@gmail.com

Az előadás címe

ROLE OF CXCR4-CXCL12 SIGNALING IN COLONIZATION OF DEVELOPING BURSA OF FABRICIUS

Szerző(k) neve és munkahelye

Viktória Halasy, Nóra Fejszák, Tamás Kovács, Lili Orbán, Nándor Nagy

Laboratory of Stem Cells and Experimental Embryology, Department of Anatomy, Histology and Embryology, Faculty of Medicine, Semmelweis University, Budapest, Hungary

Bemutatás módja

Poszter

Szekció

Poster presentations: Immunology

Language of the presentation

Hungarian

Témacsoport

molecular sciences

Összefoglaló szövege

Introduction: The bursa of Fabricius (BF) is a central lymphoid organ of the birds responsible for the B cell maturation and the antigen specific IgM-IgG switch. In the developing embryo
B cell precursors migrate to the epithelio-mesenchymal rudiment of the BF where they proliferate and diversify their B cell receptor repertoire. In mammals the presence of CXCR4, a receptor for the chemokine stromal cell-derived factor-1 (SDF1, also named CXCL12) mediates migration of leukocytes and hematopoietic progenitors in response to CXCL12. Recent microarray work revealed different expression levels of CXCR4/CXCL12 signaling in developing avian B cells.
Aims: The first aim of this work was to analyze the normal expression pattern of CXCR4 and CXCL12 during development of the BF. To determine whether the CXCR4/CXCL12 signaling mediates migration of avian B cell precursors we have investigated the effect of inhibiting endogenous CXCR4 signaling with AMD3100.
Methods: GFP-chicken chorioallantoic membrane (CAM) assays, in situ hybridization, immunocytochemistry and immunofluorescence technique.
Results: CXCR4 receptor is first expressed by B cell progenitors in the BF at embryonic
day 10 (E10) and show an increasing expression of the receptor from the B cell immigration until hatch. After hatching CXCR4 expression is slowly downregulating from chB6+ medullary B cells. CXCL12 mRNA expression is detected in the BF mesenchyme during all stages of B cell colonization: earliest CXCL12 expression was observed at E10 in the subepithelial mesenchyme. As lymphoid cells colonize the surface epithelium and induce follicle bud formation CXCL12 expression decrease from the mesenchyme and becomes more pronounced inside the developing follicles. Analysis of CAM showed considerable reduction of B cells in follicle buds.
Conclusion: Our results demonstrate that the CXCR4/CXCL12 pathway represent a significant signal for the migration of the B cell precursors into the BF primordium and the colonization of the bursal follicles.

Grant NFKI: 124740

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4129

Presentation file URL

//docs.kmcongress.com/phd2019/eposter_6052.jpeg

Authors (legacy)

Viktória Halasy, Nóra Fejszák, Tamás Kovács, Lili Orbán, Nándor Nagy

Laboratory of Stem Cells and Experimental Embryology, Department of Anatomy, Histology and Embryology, Faculty of Medicine, Semmelweis University, Budapest, Hungary

Data of the presenter

Doctoral School: Semmelweis University, Doctoral School of Molecular Medicine
Program: Embryology, Theoretical, Experimental and Clinical Developmental Biology
Supervisor: Nándor Nagy
E-mail address: viktoria.halasy@gmail.com
poster