PhD Scientific Days 2020

Molecular Sciences IV. Posters

Investigation of the Interaction Between Nutrient Supply and Circadian Rhythm in the Model Organism Neurospora Crassa

Előadó neve

Szőke, Anita, MSc

Előadó munkahelye

Department of Physiology, Semmelweis University

Előadó telefonszáma

003614591500/60412

Előadó e-mail címe

szoke.anita@med.semmelweis-univ.hu

Az előadás címe

Investigation of the Interaction Between Nutrient Supply and Circadian Rhythm in the Model Organism Neurospora Crassa

Szerző(k) neve és munkahelye

Anita Szőke1, Norbert Gyöngyösi2 and Krisztina Káldi1

1Department of Physiology, Semmelweis University, Budapest
2Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University, Budapest

Szekció

Molecular Sciences IV. Posters

Language of the presentation

Hungarian

Section, first choice

Molecular Sciences

Section, second choice

Health Sciences

Összefoglaló szövege

Introduction
Increasing experimental and clinical data suggest the significance of the interplay between metabolism and circadian timekeeping. Metabolic compensation allows the circadian oscillator to keep a nearly constant period in an environment with changing substrate levels. On the other hand, nutrient availability and coupled metabolic conditions are important Zeitgebers affecting the circadian phase of various physiological processes.

Aims
In a previous study we examined the role of the RAS2 protein in the metabolic compensation of the circadian rhythm in the model organism Neurospora crassa. As RasGEFs are upstream regulators of RAS-mediated pathways, in our next study we aimed to characterize the circadian phenotype of a strain deficient in the expression of a putative rasgef under various nutrient levels.

Method
The conidiation rhythm was analysed in race tube assay under various conditions. The promoter activity was detected in luciferase assay. The expression levels of the investigated genes were determined by real-time PCR measurements following RNA isolation.

Results
The conidiation rhythm of the rasgef deficient strain shows pronounced metabolic sensitivity. In race tube assays the phase of conidiation is significantly delayed both in light-dark and temperature cycles. Under constant conditions the period is slightly affected by the mutation. When a luciferase reporter was expressed under the control of the rasgef promoter, rhythmic activity was detected suggesting that rasgef is a clock-controlled gene (ccg). In addition, expression levels of rasgef were sensitive to the glucose content of the medium.

Conclusion
In summary, we suggest that rasgef is a novel glucose repressible clock-controlled gene (ccg) which may play an important role in the metabolic regulation of the circadian clock of Neurospora crassa.

SUPPORTED BY THE ÚNKP-19-3-IV-SE-3 NEW NATIONAL EXCELLENCE PROGRAM OF THE MINISTRY FOR INNOVATION AND TECHNOLOGY.

Additional Information

Krisztina Káldi
kaldi.krisztina@med.semmelweis-univ.hu

Bemutatás módja

Poszter

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

2921

Start

12:40

End

12:43

Authors (legacy)

Anita Szőke1, Norbert Gyöngyösi2 and Krisztina Káldi1

1Department of Physiology, Semmelweis University, Budapest
2Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University, Budapest