PhD Scientific Days 2020

Neurosciences I. Posters

Neurochemical and Electrophysiological Features of Extended Amygdala Projecting Neurons Located in the Periaqueductal Grey and Dorsal Raphe Nucleus

Előadó neve

Müller, Kinga

Előadó munkahelye

Institute of Experimental Medicine

Előadó telefonszáma

+36-70-775-6688

Előadó e-mail címe

muller.kinga@koki.mta.hu

Az előadás címe

Neurochemical and Electrophysiological Features of Extended Amygdala Projecting Neurons Located in the Periaqueductal Grey and Dorsal Raphe Nucleus

Szerző(k) neve és munkahelye

Kinga Müller1, Laura Rovira-Esteban1, Filippo Weisz1, Norbert Hájos1

1 Institute of Experimental Medicine, Budapest

Szekció

Neurosciences I. Posters

Language of the presentation

English

Section, first choice

Neurosciences

Section, second choice

Neurosciences

Összefoglaló szövege

Periaqueductal grey (PAG) and dorsal raphe nucleus (DRN) are brainstem structures, which are involved in defensive responses, nociception or social interaction. The vPAG/DRN dopaminergic neurons project to the extended amygdala, particularly to the lateral part of the central amygdala (CeL) and to the bed nucleus of stria terminalis (BNST). Vasoactive intestinal polypeptide (VIP) and cholecystokinin (CCK) containing cells, which can be found in this region, also innervate both CeL and BNST. Given the confounding and partially overlapping information available on the cytoarchitecture and projections of the vPAG/DRN nuclei, we aimed to explore the neurochemical content of vPAG/DRN neurons, focusing on those ones, which project to CeL and/or BNST.
To this end, we intracranially injected retrograde tracers into the BNST and CeL of transgenic mice, to visualize the projecting cells and identify their neurochemical content using immunocytochemistry. Our results indicate a wider distribution of CCK+ neurons within the whole PAG. In contrast, VIP+ neurons are confined to the lateral and ventral parts adjacent to the 4th ventricle. We found that 39% of VIP+ neurons co-expressed CCK. In tracing experiments using CCK-DsRed mice (n=2) we revealed that more than 60% of projecting cells in vPAG/DRN contained only CCK, 2% contain only VIP and 25% express both neuropeptides. We found 13.3% of cells projecting to both nuclei.
Using in vitro whole-cell patch clamp technique in vPAG/DRN containing sections of VIP-ZsGreen1 mice, we recorded the firing pattern of VIP+ neurons and analyzed their intrinsic properties, which where compared to those data obtained from inhibitory neurons sampled in VGAT-ZsGreen1 mice. Our data show that vPAG/DRN VIP+ neurons have similar features than those described for dopaminergic neurons, as well as clearly different membrane properties than local inhibitory neurons.
In summary, we provide a map for the distribution of CCK and VIP containing neurons, as well as the overlap in their neurochemical content in vPAG/DRN. Based on the electrophysiological features of these VIP+ cells likely glutamatergic. We demonstrate that both CCK+ and VIP+ neurons project to the CeL and BNST, and interestingly that some projecting cells send collaterals to both regions, providing the structural basis for the simultaneous control of the CeL and BNST function.

Additional Information

Norbert Hájos
hajos.norbert@koki.hu

Bemutatás módja

Poszter

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4630

Start

12:44

End

12:47

Authors (legacy)

Kinga Müller1, Laura Rovira-Esteban1, Filippo Weisz1, Norbert Hájos1

1 Institute of Experimental Medicine, Budapest