PhD Scientific Days 2020

Molecular Sciences III. Posters

Beneficial Effects of Tricetin Flavonoid in Cerulein Induced Acute Pancreatitis in Mice

Előadó neve

Máté, Nagy-Pénzes

Előadó munkahelye

Department of Medical Chemistry, University of Debrecen

Előadó telefonszáma

06305679499

Előadó e-mail címe

nagy.mate@med.unideb.hu

Az előadás címe

Beneficial Effects of Tricetin Flavonoid in Cerulein Induced Acute Pancreatitis in Mice

Szerző(k) neve és munkahelye

Máté Nagy-Pénzes1, Zoltán Hajnády1, Zsolt Regdon1, László Virág1
1 Department of Medical Chemistry, University of Debrecen

Szekció

Molecular Sciences III. Posters

Language of the presentation

Hungarian

Section, first choice

Molecular Sciences

Section, second choice

Pharmaceutical Sciences

Összefoglaló szövege

INTRODUCTION
Acute (AP) and the chronic pancreatitis (CP) are one of the most common gastrointestinal diseases of the developed societies, with 30-40% mortality. The reason of the high mortality is the lack of specific therapy, wich could reduce the progression of the diseases. In physiological condition the digestive enzymes are synthetized in zymogen granules in pancreatic acinar cells. Due to some insults, the enzymes are prematurely activated in the cells, from where they harm the tissues of the organ. In case of long term AP evolves the chronic form with multiorgan failure and diabetes mellitus because of a severe pancreatic injury.

AIMS
Our aim was to examine the possible beneficial effect of an antioxidant flavonoid (tricetin) in the severity of the cerulein induced AP.

METHOD
In vitro the effect of the flavonoid was investigated in cerulein treated (in 100nM concentracion) isolated acinar cells. Calcein, LDH, PI assays, cellomics, and qPCR were used in the examination.
After that we investigated the effect of tricetin in vivo by intraperitoneal injection (10mg/kgBW) 1 hour before the AP was induced by intraperitoneal injections of cerulein (50μg/kgBW) eight times at 1 hour intervals. The mice were sacrificed 10 hours after the fist cerulein injection. We exemined α-amylase and lipase level of the serum, and MPO level from the pancreas. We made HE staining sections and qPCR from the pancreatic tissues.

RESULTS
In vitro the flavonoid significantly (α=0,05) decreased the harmful effect of the cerulein treatment. In vivo it
significantly (α=0,05) derceased the level of the digestive enzymes and the MPO and also decreased the expression of inflammatory genes.

CONCLUSION
Our results show that used tricetin successfully decreased the harmful effect of the cerulein in isolated acinar cells, and derceased the severity of the cerulein induced acute pancreatitis in mice.

Additional Information

Supervisor: László Virág
E-mail address: lvirag@med.unideb.hu

Bemutatás módja

Poszter

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4644

Start

11:16

End

11:19

Authors (legacy)

Máté Nagy-Pénzes1, Zoltán Hajnády1, Zsolt Regdon1, László Virág1
1 Department of Medical Chemistry, University of Debrecen