PhD Scientific Days 2020

Molecular Sciences II. Posters

The Role of Neutrophil Granulocytes in Skeletal Muscle Regeneration

Előadó neve

Halász, Hajnalka Emese, MSc

Előadó munkahelye

University of Debrecen, Faculty of Medicine, Department of Immunology

Előadó telefonszáma

+36 52 417 159, 65104

Előadó e-mail címe

halasz.hajnalka@med.unideb.hu

Az előadás címe

The Role of Neutrophil Granulocytes in Skeletal Muscle Regeneration

Szerző(k) neve és munkahelye

Author and co-authors: Hajnalka Emese Halász1,2, Tamás Varga3, Péter Gogolák1
Affiliation details:
1Department of Immunology, Faculty of Medicine, University of Debrecen
2University of Debrecen, Doctoral School of Molecular Cell and Immune Biology
3Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen

Szekció

Molecular Sciences II. Posters

Language of the presentation

Hungarian

Section, first choice

Molecular Sciences

Section, second choice

Molecular Sciences

Összefoglaló szövege

Introduction: The skeletal muscle is permanently exposed to physical damage but in normal condition the tissue is able to repair itself in a very efficient way. However, we know several degenerative muscle diseases with inflammation and impaired tissue regeneration. Neutrophils enter traumatized, stressed tissues firstly and act as professional phagocytic cells. Like macrophages, neutrophils also form a heterogeneous population, they can contribute to inflammation and to repair as well. The myeloid-specific deletion of the Mcl-1 antiapoptotic protein in mice leads to dramatic reduction of circulating and tissue neutrophil counts without obvious changes in other immune cell numbers. In these Mcl-1 deficient mice we observed a delayed skeletal muscle regeneration which may indicate the role of neutrophils in the repair mechanisms.
Aims: Our aim is to investigate the role of neutrophils during a sterile muscle injury model. We would like to identify the neutrophil subtypes in the tissue regeneration in order to understand the molecular mechanisms and immunological pathways better.
Methods: The muscle injury is induced by cardiotoxin injection in tibialis anterior muscle, both in Mcl-1 KO and wild-type mice. The muscle isolation and processing is performed at day 1, 2, 4 and 8 post injury. The muscle regeneration is followed by analysing the satellite cells and infiltrating immune cells with flow cytometry. We would like to restore the delayed repair with transferring Ly6G+ neutrophils from MHC-compatible, WT mice by adoptive cell transfer.
Results: The migration of macrophages into the damaged muscle was delayed in the absence of neutrophils. The number of Ly6ChighF4/80med and Ly6CmedF4/80high macrophages differed in Mcl-1 KO mice compared to the control mice. We optimized the appropriate neutrophil cell number for the adoptive cell transfer and showed that the transferred Ly6G+ CD45.1 neutrophils entered the damaged muscle tissue after 24 hours.
Conclusion: These preliminary results show that the neutrophils have a pivotal role in normal muscle regeneration. The identification and investigation of neutrophil subtypes during a sterile muscle injury might be useful in the discovery of new therapeutic targets in muscle degenerative disorders and injuries.
Funding: This work is supported by NKFIH K-125477, EFOP-3.6.3-VEKOP-16-2017-00009.

Additional Information

Supervisor: Péter Gogolák
E-mail: gogy@med.unideb.hu

Support for conference attendance: EFOP-3.6.3-VEKOP-16-2017-00009.

Bemutatás módja

Poszter

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4654

Start

12:53

End

12:56

Authors (legacy)

Author and co-authors: Hajnalka Emese Halász1,2, Tamás Varga3, Péter Gogolák1
Affiliation details:
1Department of Immunology, Faculty of Medicine, University of Debrecen
2University of Debrecen, Doctoral School of Molecular Cell and Immune Biology
3Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen