PhD Scientific Days 2020

Theoretical and Translational I. Posters

Novel Aspects of the Complex Cannabinoid Signaling in Human Sebocytes

Előadó neve

Tóth, Kinga Fanni, MSc

Előadó munkahelye

University of Debrecen, Faculty of Medicine, Department of Physiology

Előadó telefonszáma

+3630 947 5907

Előadó e-mail címe

toth.kinga.fanni@med.unideb.hu

Az előadás címe

Novel Aspects of the Complex Cannabinoid Signaling in Human Sebocytes

Szerző(k) neve és munkahelye

Kinga Fanni Tóth 1,2
József Arany 1,2
Dorottya Ádám 1,2
Christos C. Zoubulis 3
Attila Oláh 1

1 Department of Physiology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary
2 Doctoral School of Molecular Medicine, University of Debrecen, Debrecen, Hungary
3 Departments of Dermatology, Venereology, Allergology and Immunology, Dessau Medical Center, Brandenburg Medical School Theodor Fontane, Dessau, Germany

Szekció

Theoretical and Translational I. Posters

Language of the presentation

Hungarian

Section, first choice

Molecular Sciences

Section, second choice

Theoretical and Translational Medicine

Összefoglaló szövege

Introduction
Sebaceous glands (SGs) are key contributors to cutaneous homeostasis. Although our understanding of their biology has grown, several aspects of human SG (patho)physiology are still unknown. Considering that SG dysfunction-associated diseases and skin conditions, such as acne, are highly prevalent disorders, there is an unmet need to explore unknown regulators of the SG physiology. We have previously shown that the “classical” endocannabinoid signaling was a positive regulator of the sebaceous lipogenesis, whereas (-)-cannabidiol (the most important non-psychotropic phytocannabinoid) exerted complex cellular anti-acne effects. Of great importance, some of its effects were mediated via the activation of non-classical cannabinoid targets (e.g. adenosine A2A receptors), highlighting that certain members of the purinergic signaling system may contribute to the regulation of SG biology.

Aims
Within the confines of the current project, we aimed to explore the role of the purinergic branch of the non-classical cannabinoid signaling in controlling sebocyte functions.
Methods
Viability, proliferation, lipid synthesis, and gene expression of human immortalized SZ95 sebocytes were monitored by MTT-assay, CyQUANT-assay, Nile Red labeling, and Q-PCR, respectively.

Results
First, we assessed the adenosine receptor expression profile of human sebocytes. Importantly, we showed that, besides A2A, A1, and A2B adenosine receptors are also expressed, whereas expression of A3 was around the detection limit. Next, we assessed the effects of a wide concentration range (1 nM – 1 mM) of adenosine. We found that it had no effect on the viability and the basal, homeostatic sebaceous lipid synthesis (24-48-hr treatments), but it significantly suppressed the arachidonic acid-induced, acne-mimicking, excessive lipogenesis (1-10 nM; 24-48 hrs), and exerted anti-proliferative effects (1 mM; 24-72 hrs).

Conclusions
Our current findings strongly argue that purinergic signaling may be involved in the regulation of SG biology. Moreover, our data highlight the possibility that certain adenosine receptors may be promising anti-acne targets.

Acknowledgement
K.F.T.’s work was supported by the New National Excellence Program of the Ministry for Innovation and Technology (ÚNKP-19-3-I-DE-141), as well as by EFOP-3.6.3-VEKOP-16-2017-00009.

Additional Information

Supervisor: Attila Oláh
E-mail address: olah.attila@med.unideb.hu

Due to official obligations, I can only attend the conference on March 17-18. Thus, I would be most grateful if you could schedule my presentation to either March 17 or 18.

K.F.T.’s work was supported by the New National Excellence Program of the Ministry for Innovation and Technology (ÚNKP-19-3-I-DE-141), as well as by EFOP-3.6.3-VEKOP-16-2017-00009.

Bemutatás módja

Poszter

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4659

Start

11:29

End

11:32

Authors (legacy)

Kinga Fanni Tóth 1,2
József Arany 1,2
Dorottya Ádám 1,2
Christos C. Zoubulis 3
Attila Oláh 1

1 Department of Physiology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary
2 Doctoral School of Molecular Medicine, University of Debrecen, Debrecen, Hungary
3 Departments of Dermatology, Venereology, Allergology and Immunology, Dessau Medical Center, Brandenburg Medical School Theodor Fontane, Dessau, Germany