PhD Scientific Days 2020

Theoretical and Translational I. Lectures

Prognostic Importance of Brain-Derived Neurotrophic Factor (BDNF) in Renal Transplantation

Előadó neve

Dr. Molnar, Agnes

Előadó munkahelye

Semmelweis University 1st Department of Pediatrics

Előadó telefonszáma

+36706229304

Előadó e-mail címe

agnes.molnar.md@gmail.com

Az előadás címe

Prognostic Importance of Brain-Derived Neurotrophic Factor (BDNF) in Renal Transplantation

Szerző(k) neve és munkahelye

Agnes Molnar (1,4), Edgar Szkibinszkij (1,2), Lilla Lenart (1,4), Adam Hosszu (1,4), Illes Kovacs (3), Laszlo Wagner (2), Attila J. Szabo (4,5) Andrea Fekete (1,4)
1 MTA-SE Lendület Diabetes Research Group, Budapest
2 SE Department of Transplantation and Surgery, Budapest
3 SE Department of Ophthalmology, Budapest
4 SE 1st Department of Paediatrics, Budapest
5 MTA-SE Pediatrics and Nephrology Research Group

Szekció

Theoretical and Translational I. Lectures

Language of the presentation

English

Section, first choice

Theoretical and Translational Medicine

Section, second choice

Clinical Medicine

Összefoglaló szövege

Introduction: The prevalence of end-stage renal disease (ESRD) has increased ten times higher in the past twenty years, where renal replacement therapy (dialysis or kidney transplantation (KTx)) is the sole treatment. KTx is the preferred option as it is associated with improved survival and quality of life as well. Delayed graft function (DGF) is one of the main problem affecting long-term kidney survival. Brain-derived neurotrophic factor (BDNF) signalling pathways play pivotal role in mitigating cerebral ischemia/reperfusion injury (IRI), however the relation of BDNF and IRI in KTx is unknown.
Aims: The aim of our human clinical study was to explore the relationship between serum BDNF concentration, BDNF gene polymorphism and renal graft function after KTx.
Method: Study characteristics: 59 ESRD patients (57% male) with average age of 54.8±12 years received KTx. Average cold ischemic time: 927±310 min, warm ischemic time: 54.5±39 min. DGF: 5 cases. Baseline triple immunosuppression therapy: tacrolimus, mycophenolate or everolimus, and prednisolone. Until now, 44 patients completed the 2 years follow-up. For a comparable control group, we collected blood samples from 79 healthy volunteers with average age of 53.9±16 years and with male gender proportion of 52%. Serum BDNF, creatinine, blood urea nitrogen, haemoglobin, blood glucose level and thrombocyte numbers were measured, before KTx, and 1 week, 1-, 3-, 6 months, and 1-, 2 years after, as well as in controls. GFR was estimated based on CKD-EPI formula. BDNF Val66Met polymorphism was determined by PCR-RFLP.
Results: There was no difference in genotype or allele distribution between any of the groups, and no correlation could be observed between serum BDNF and different genotypes either. Serum BDNF level was lower in ESRD patients than healthy controls (p=0.03). There was a weak correlation and marginal significance (p=0.056) between eGFR and serum BDNF level in controls, while in KTx recipients this correlation reached higher significance (p=0.01). Above median BDNF values at 1 month after KTx were predictive for better graft function during the 2 observed years.
Conclusion: Our preliminary human study proposes that BDNF could be a novel biomarker of posttransplant graft function, however further clinical studies with significantly larger population are definitely needed to confirm these results.

Additional Information

Grants: LP008/2017, OTKA-K112629, NNOTKA-11460, VKE-2017-00006, FK124491, EEMOFAKT-2017, SUPPORTED BY THE ÚNKP-19-3-I-SE-79 NEW NATIONAL EXCELLENCE PROGRAM OF THE MINISTRY FOR INNOVATION AND TECHNOLOGY.

Supervisor: Andrea Fekete
E-mail address: fekete.andrea@med.semmelweis-univ.hu

Bemutatás módja

Szóbeli

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4668

Start

11:05

End

11:20

Authors (legacy)

Agnes Molnar (1,4), Edgar Szkibinszkij (1,2), Lilla Lenart (1,4), Adam Hosszu (1,4), Illes Kovacs (3), Laszlo Wagner (2), Attila J. Szabo (4,5) Andrea Fekete (1,4)
1 MTA-SE Lendület Diabetes Research Group, Budapest
2 SE Department of Transplantation and Surgery, Budapest
3 SE Department of Ophthalmology, Budapest
4 SE 1st Department of Paediatrics, Budapest
5 MTA-SE Pediatrics and Nephrology Research Group