PhD Scientific Days 2020

Molecular Sciences I. Lectures

Alterations in Titin Expression in Athlete’s Heart Using a Rat Model

Előadó neve

Dr. Kellermayer, Dalma

Előadó munkahelye

Heart and Vascular Center, Semmelweis University

Előadó telefonszáma

+36204156011

Előadó e-mail címe

dalmakeller@gmail.com

Az előadás címe

Alterations in Titin Expression in Athlete’s Heart Using a Rat Model

Szerző(k) neve és munkahelye

Dalma Kellermayer1, Attila Oláh1, Mihály Ruppert1, Bálint András Barta1, Alex Ali Sayour1, Eszter Virág-Tulassay1, Miklós Kellermayer2, Béla Merkely1, Tamás Radovits1

1Heart and Vascular Center, Semmelweis University, Budapest, Hungary
2 Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary

Szekció

Molecular Sciences I. Lectures

Language of the presentation

English

Section, first choice

Molecular Sciences

Section, second choice

Clinical Medicine

Összefoglaló szövege

Introduction: The giant elastic protein titin provides passive stiffness to striated muscles. The adult cardiac muscle contains two titin isoforms: the more compliant N2BA and the stiffer N2B isoforms. Titin reduces passive stiffness in cardiac muscle by increased expression of the more compliant N2BA isoform (elevated N2BA:N2B ratio). Moreover, decreased passive stiffness is associated with increased exercise tolerance. Long-term, chronic exercise induces physiological adaptation of the heart, termed athlete’s heart. Currently, there is limited data of titin’s role in the athlete’s heart.
Aims: Our aim is to evaluate exercise-induced morphological and functional changes of the heart. Furthermore, to determine the N2BA:N2B ratio in the rat model of athlete’s heart.
Methods: Rats were divided into exercised (n=12) and control (n=12) groups. Athlete’s heart was induced by a 12-week-long swim training (200min/day). The control group swam 5 min/day. Following the training period cardiac changes were assessed by echocardiography. Left ventricular (LV) pressure-volume (P-V) analysis was performed to examine in vivo cardiac function. Titin isoform expressions were detected by sodium-dodecyl-sulfate (SDS)-agarose gel electrophoresis.
Results: Echocardiography and post-mortem measured cardiomyocyte diameters confirmed LV hypertrophy in exercised rats. P-V analysis showed improved contractility, active relaxation and mechanoenergetics in the exercised group. The N2BA:N2B titin ratio was significantly increased in exercised rats compared to controls.
Conclusions: Our results confirm the morphological and functional changes of the athlete’s heart. The increased ratio of N2BA:N2B titin corresponds to a more compliant heart in the exercised rats.
Funding: Supported by the National Research, Development and Innovation Office of Hungary (K 120277) and the ÚNKP-19-3-I New National Excellence Program of The Ministry For Innovation and Technology.

Additional Information

Supervisor: Radovits Tamás
E-mail address: radovitstamas@yahoo.com

Bemutatás módja

Szóbeli

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4165

Start

11:05

End

11:20

Authors (legacy)

Dalma Kellermayer1, Attila Oláh1, Mihály Ruppert1, Bálint András Barta1, Alex Ali Sayour1, Eszter Virág-Tulassay1, Miklós Kellermayer2, Béla Merkely1, Tamás Radovits1

1Heart and Vascular Center, Semmelweis University, Budapest, Hungary
2 Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary