PhD Scientific Days 2020

Theoretical and Translational II. Lectures

Examination of the Relation between Red Blood Cell Aggregation and Hematocrit in Various Species

Előadó neve

Baráth, Barbara, MSc

Előadó munkahelye

Department of Operative Techniques and Surgical Research, Faculty of Medicine, University of Debrecen, Debrecen, Hungary

Előadó telefonszáma

06308624888

Előadó e-mail címe

barath.barbara@med.unideb.hu

Az előadás címe

Examination of the Relation between Red Blood Cell Aggregation and Hematocrit in Various Species

Szerző(k) neve és munkahelye

1. Barbara Baráth, Department of Operative Techniques and Surgical Research, Faculty of Medicine,
University of Debrecen, Debrecen, Hungary

2. Viktória Somogyi, Department of Operative Techniques and Surgical Research, Faculty of Medicine,
University of Debrecen, Debrecen, Hungary

3. Bence Tánczos, Department of Operative Techniques and Surgical Research, Faculty of Medicine,
University of Debrecen, Debrecen, Hungary

4. Norbert Németh, Department of Operative Techniques and Surgical Research, Faculty of Medicine,
University of Debrecen, Debrecen, Hungary

5. Ádám Deák, Department of Operative Techniques and Surgical Research, Faculty of Medicine,
University of Debrecen, Debrecen, Hungary

Szekció

Theoretical and Translational II. Lectures

Language of the presentation

English

Section, first choice

Theoretical and Translational Medicine

Section, second choice

Health Sciences

Összefoglaló szövege

Background: Red blood cell (RBC) aggregation is a reversible clumping of cells at low shear-rate or in stasis. In addition to cellular and plasmatic factors, hematocrit (Hct) also determines this process, since the number of cells involved has an effect on the degree of aggregation and the dynamics of cell-cell interactions.
Aims: We hypothesized that the RBC aggregation – hematocrit relation differs in species.
Methods: Blood samples were taken from 7 healthy male volunteers (DE-RKEB-3189-2010), 6 male Crl:WI rats, 6 male Beagle dogs, and 7 female Hungahib pigs (24/2016/UDCAW, 7/2014/UDCAW, 13/2014/UDCAW). From the samples 20, 40 and 60 % Hct RBC - autologous plasma suspensions were prepared. We measured hematological parameters (Sysmex F-800 or Sysmex K-4500 microcell counter), RBC aggregation by the light-transmission (Myrenne MA-1 aggregometer) and light-reflectance method (LoRRca Maxsis Osmoscan ektacytometer). For statistical analysis, ANOVA test was used.
Results: Using the light-transmission method, the M5s aggregation index parameter significantly differed between the species. At 20% hematocrit human values were significantly higher than pigs’ values, at 40% Hct the rat blood samples showed a significantly lower results compared to all other species. In the LoRRca aggregation index for human, dog and pig samples there was a decrease with the risen of Hct values. At 60% human specimens were significantly lower and rat samples were significantly higher than the other samples. In the aggregation amplitude (Amp) the lowest values were measured in rat, followed by pig, human and dog blood samples. At 20% human samples differed from all other species. At 60% the rat samples showed lower Amp values to the other. In the syllectogram half-life (t1/2) parameter, in the native samples the dog blood showed the highest values. In human, dog and pig samples, there was a continuous increase in t1/2. The highest increase was observed in human. At 60% Hct the human and the dog specimens showed a difference to the other samples, both were higher than the others.
Conclusions: The relationship between RBC aggregation and hematocrit varies between species. Results suggest that the ability of human blood to aggregate is the most sensitive to hematocrit’s changes. These data can be useful for extrapolating results of animal experiments.

Additional Information

Supervisor : Ádám Deák, D.V.M, PhD
E-mail address: deak.adam@med.unideb.hu
EFOP-3.6.3-VEKOP-16-2017-00009 DE

Bemutatás módja

Szóbeli

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4676

Start

11:50

End

12:05

Authors (legacy)

1. Barbara Baráth, Department of Operative Techniques and Surgical Research, Faculty of Medicine,
University of Debrecen, Debrecen, Hungary

2. Viktória Somogyi, Department of Operative Techniques and Surgical Research, Faculty of Medicine,
University of Debrecen, Debrecen, Hungary

3. Bence Tánczos, Department of Operative Techniques and Surgical Research, Faculty of Medicine,
University of Debrecen, Debrecen, Hungary

4. Norbert Németh, Department of Operative Techniques and Surgical Research, Faculty of Medicine,
University of Debrecen, Debrecen, Hungary

5. Ádám Deák, Department of Operative Techniques and Surgical Research, Faculty of Medicine,
University of Debrecen, Debrecen, Hungary