PhD Scientific Days 2020

Molecular Sciences II. Posters

Neuroimmune Communication in the Epidermal Compartment of Human Skin

Előadó neve

Horváth, Dorottya, MSc

Előadó munkahelye

University of Debrecen, Faculty of Medicine, Department of Immunology

Előadó telefonszáma

+36 52 417 159, 65434

Előadó e-mail címe

horvath.dorottya@med.unideb.hu

Az előadás címe

Neuroimmune Communication in the Epidermal Compartment of Human Skin

Szerző(k) neve és munkahelye

Dorottya Horváth1, Zsófia Pénzes1, Shahrzad Alimohammadi1, Tamás Bíró1,2, Attila Gábor Szöllősi1

Affiliation details:
1Department of Immunology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary; Doctoral School of Molecular Medicine, University of Debrecen, Debrecen, Hungary.
2Hungarian Center of Excellence for Molecular Medicine, Szeged, Hungary.

Szekció

Molecular Sciences II. Posters

Language of the presentation

Hungarian

Section, first choice

Molecular Sciences

Section, second choice

Molecular Sciences

Összefoglaló szövege

The immune system is strongly linked to the nervous system within the human organism, to the point that in many diseases they cannot be fragmented to separate entities. Specifically, some inflammatory skin diseases such as atopic dermatitis and psoriasis have been reported to be intensified by stress, and, interestingly the skin lesions in these diseases sometimes resolve after nerve injury. The putative cause underlying this empirical observation is that sensory nerves secrete neuropeptides and other mediators that are important members of neuro-immune communication. Neuropeptides play a key role in skin immunity, the inflammation process, and wound healing. Inside the epidermis, the only dedicated resident immune cells under steady-state are the antigen-presenting cells called Langerhans cells (LC). LCs are anatomically associated with neurons that produce, Calcitonin Gene-Related Peptide (CGRP) possibly playing a role in the cutaneous inflammation.
Previous reports have shown that CGRP reduces LC antigen-presentation to a Th1 clone however, the exposure of the CGRP in LCs enhances the antigen-presentation for Th2 clones. The cytokine secretion after chicken ovalbumin (cOVA) challenge was shifted toward a Th2 profile, since IL-4 was increased while IFNγ production was decreased. While these results are interesting, they were carried out in mouse models, therefore we decided to test all the results on human monocyte-derived LCs.
In this project, the aim is to discover the communication between LCs and neurons located in the skin.
As a first, exploratory step we utilized RNA sequencing from monocyte-derived LCs samples from five donors and we detected the expression of neuropeptide receptor genes. We found a high expression of CGRP receptor and its coreceptor RAMP1 and also one of the neurotensin receptors (SORT1, also known as NTR3) and the Brain Natriuretic peptide receptor(NPR1). These results will be validated with q-PCR and western-blot in monocyte-derived LCs. We would like to analyze the effect of these peptides in the differentiation of monocyte-derived LCs and dendritic cells with flow cytometry. Furthermore, we are also curious about the signal transduction pathways initiated by these peptides.

Additional Information

Supervisor: Attila Gábor Szöllősi
email: szollosi.attila@med.unideb.hu

Funding: EFOP-3.6.3-VEKOP-16-2017-00009

Bemutatás módja

Poszter

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4680

Start

12:59

End

13:02

Authors (legacy)

Dorottya Horváth1, Zsófia Pénzes1, Shahrzad Alimohammadi1, Tamás Bíró1,2, Attila Gábor Szöllősi1

Affiliation details:
1Department of Immunology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary; Doctoral School of Molecular Medicine, University of Debrecen, Debrecen, Hungary.
2Hungarian Center of Excellence for Molecular Medicine, Szeged, Hungary.