PhD Scientific Days 2020

Molecular Sciences III. Posters

The Role of Phytocannabinoids on Monocyte-derived Dendritic Cells Differentiation and Maturation

Előadó neve

Pénzes, Zsófia

Előadó munkahelye

Department of Immunology, University of Debrecen

Előadó telefonszáma

+36-20-923-1356

Előadó e-mail címe

penzeszsofia@gmail.com

Az előadás címe

The Role of Phytocannabinoids on Monocyte-derived Dendritic Cells Differentiation and Maturation

Szerző(k) neve és munkahelye

Zsófia Pénzes1,2, Shahrzad Alimohammadi1,2, Dorottya Horváth1,2, Tamás Bíró1,3, Attila Gábor Szöllősi1,

1Department of Immunology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
2Doctoral School of Molecular Medicine, University of Debrecen, Debrecen, Hungary.
3Hungarian Center of Excellence for Molecular Medicine, Szeged, Hungary.

Szekció

Molecular Sciences III. Posters

Language of the presentation

Hungarian

Section, first choice

Molecular Sciences

Section, second choice

Theoretical and Translational Medicine

Összefoglaló szövege

Introduction
Dendritic cells (DCs) act as a major link between the innate and adaptive immune systems. Their main function is to capture antigens in the periphery (e.g. the skin) and to present them to T cells, and to thereby act as messengers between the innate and the adaptive immune systems. Nowadays, as the use of herbal active ingredients from the cannabis plant (Cannabis sativa) is gaining in popularity, the use of these products has shown an exponential increase, even in topical formulations that can directly influence the main function of DCs.
Up to now more than 100 different cannabinoids have been isolated from the Cannabis plant, which are called phytocannabinoids (PCs). PCs, especially the non-psychoactive compounds, have been shown to have beneficial effects in multiple inflammatory disease models; nevertheless, they were mostly investigated in mouse models and not on human cells.
Method
In our current work, we investigated the effect of non-psychoactive PCs (Cannabidiol (CBD), Cannabinol (CBN), Cannabigerol (CBG), Tetrahydrocannabivarin (THCV), in regulating responses of monocyte-derived dendritic cells (moDCs) by monitoring changes in their maturation markers.
Results
First, to exclude the possibility of the onset of early apoptotic or necrotic processes, we performed viability tests (PrestoBlue and G6PDH release assay), which demonstrated that our phytocannabinoids did not cause any cell death of moDC.
Moreover, we found that our PCs generally induced cell maturation at high concentrations on moDC, whereas LPS-induced maturation was not affected.
To support our functional data we showed that Cannabinoid receptor 1 is expressed on the protein level with the help of western blots.
Furthermore, we also found that CBG and THCV lead to an increase in the pinocytotic capability of iDCs. We also investigated the effect of PCs on TRPV channels by monitoring changes in the intracellular Ca2+ concentration.
To explore the secondary messenger systems activated by PC treatment we determined the relative levels of human protein kinase phosphorylation with the help of Proteome Profiler Human Phospho-Kinase Array Kit.
Conclusion
Taken together, our data suggest that PCs may play varied roles on the immunological function of moDCs, and their use as potent anti-inflammatory treatments must be tested extensively.

Additional Information

Attila Gábor Szöllősi
szollosi.attila@med.unideb.hu

EFOP-3.6.3-VEKOP-16-2017-00009

Bemutatás módja

Poszter

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4681

Start

11:22

End

11:25

Authors (legacy)

Zsófia Pénzes1,2, Shahrzad Alimohammadi1,2, Dorottya Horváth1,2, Tamás Bíró1,3, Attila Gábor Szöllősi1,

1Department of Immunology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
2Doctoral School of Molecular Medicine, University of Debrecen, Debrecen, Hungary.
3Hungarian Center of Excellence for Molecular Medicine, Szeged, Hungary.