PhD Scientific Days 2020

Pathology and Oncology I. Lectures

Intratumoral Cisplatin Measurement by LA-ICP-MS: an Innovative Technique to Assess the Clinical Impact of Chemotherapeutic Drug Distribution in Malignant Pleural Mesothelioma

Előadó neve

Sólyom-Tisza, Anna, MSc

Előadó munkahelye

1st Department of Pathology and Experimental Cancer Research, Semmelweis University; Department of Tumor Biology, National Korányi Institute of Pulmonology, Budapest, Hungary

Előadó telefonszáma

+36207739471

Előadó e-mail címe

solyomtisza.anna@gmail.com

Az előadás címe

Intratumoral Cisplatin Measurement by LA-ICP-MS: an Innovative Technique to Assess the Clinical Impact of Chemotherapeutic Drug Distribution in Malignant Pleural Mesothelioma

Szerző(k) neve és munkahelye

Anna Solyom-Tisza12, Thomas Klikovits3, Max Bonta4, Mir Alireza Hoda3, Andreas Limbeck4, Balázs Hegedüs3, Walter Klepetko3, Sándor Paku2, Balázs Döme13, Viktória László13
1 Department of Tumor Biology, National Korányi Institute of Pulmonology, Budapest
2 1st Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest
3 Division of Thoracic Surgery, Medical University of Vienna, Vienna
4 Division of Instrumental Analytical Chemistry, Institute of Chemical Technologies and Analytics, Vienna University of Technology, Vienna

Szekció

Pathology and Oncology I. Lectures

Language of the presentation

English

Section, first choice

Pathology and Oncology

Section, second choice

Theoretical and Translational Medicine

Összefoglaló szövege

Introduction: Malignant pleural mesothelioma (MPM) is a devastating malignancy with dismal prognosis. In MPM the systemic chemotherapy is based on cisplatin. Although platinum agents have been applied for decades to treat solid tumors, there is limited information about the concentration and spatial distribution of platinum (Pt) in human MPM.
Aims: We aimed to analyze the spatial distribution of Pt by laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) in MPM tissues and in paired serum samples. The study attempts to clarify if insufficient drug penetration in the tumor tissue is a major component of platinum resistance in MPM.
Method: We analyzed the spatial distribution of Pt in the surgically removed tumor samples (n=27) after induction chemotherapy (CHT) with LA-ICP-MS, which enables 2D imaging. These results were compared with clinicopathological features. In addition, microvessel density (MVD) and ratio of apoptotic cells have also been investigated. With the 2D imaging we examined the heterogeneity of the tumor tissue in correlation with drug distribution. We used collagen staining to label the fibrotic tissue compartments. Additionally, we also measured the blood Pt levels and sought for potential correlations with the tissue concentrations and with the abovementioned parameters as well.
Results: We found that the spatial distribution of Pt was heterogenous in the tissue samples. Large collagen-rich fibrotic areas had high Pt levels while the tumorous compartment contained relatively low concentrations. There was no correlation between serum and tissue Pt concentrations, but the circulating drug concentrations negatively correlated with the time between the last CHT cycle and sample collection. We could not observe any correlation between tissue/serum Pt concentrations and age and number of CHT cycles received, neither between tissue MVD and Pt concentration. The Pt content of tumor tissue had no prognostic significance.
Conclusion: By using an innovative technique (LA-ICP-MS) for lateral trace element distribution analysis, we are the first to demonstrate heterogenous Pt levels in human MPM tissue samples.

Additional Information

Viktória László
E-mail address: laszlo.viktoria@gmail.com

Bemutatás módja

Szóbeli

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4684

Start

10:20

End

10:35

Authors (legacy)

Anna Solyom-Tisza12, Thomas Klikovits3, Max Bonta4, Mir Alireza Hoda3, Andreas Limbeck4, Balázs Hegedüs3, Walter Klepetko3, Sándor Paku2, Balázs Döme13, Viktória László13
1 Department of Tumor Biology, National Korányi Institute of Pulmonology, Budapest
2 1st Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest
3 Division of Thoracic Surgery, Medical University of Vienna, Vienna
4 Division of Instrumental Analytical Chemistry, Institute of Chemical Technologies and Analytics, Vienna University of Technology, Vienna