PhD Scientific Days 2020

Pharmaceutical Sciences I. Lectures

Stability Tracking of pH Modifier- or Solubilizer-Containing Furosemide-Loaded Electrospun Nanofibrous Systems

Előadó neve

Dr. Kovács, Andrea

Előadó munkahelye

Gedeon Richter Plc., Formulation R&D; University Pharmacy Department of Pharmacy Administration, Semmelweis University

Előadó telefonszáma

06302492732

Előadó e-mail címe

kovacs.andrea92@gmail.com

Az előadás címe

Stability Tracking of pH Modifier- or Solubilizer-Containing Furosemide-Loaded Electrospun Nanofibrous Systems

Szerző(k) neve és munkahelye

Andrea Kovács1,2; Adrienn Kazsoki2; Géza Jakab3; Balázs Démuth1,4; Bernadett, Szirányi1; Romána Zelkó2*

1 Gedeon Richter Plc., Formulation R&D, Gyömrői Street 19-21, H-1103 Budapest, Hungary
2 University Pharmacy Department of Pharmacy Administration, Semmelweis University, Hőgyes Endre Street 7-9, H-1092 Budapest
3 Semmelweis University, Department of Pharmaceutics, Hőgyes Endre Street 7, Budapest H-1092, Hungary.

4 Department of Organic Chemistry and Technology, Budapest University of Technology and Economics, Budafoki út 8. 3, H-1103 Budapest, Hungary

Szekció

Pharmaceutical Sciences I. Lectures

Language of the presentation

Hungarian

Section, first choice

Pharmaceutical Sciences

Section, second choice

Molecular Sciences

Összefoglaló szövege

Introduction: Electrospun nanofibrous drug delivery systems are promising alternatives for enhanced solubility formulations of drugs belonging to BCS (Biopharmaceutical Classification System) IV class, like furosemide, by keeping the drug in an amorphous state.
Aims: This study aimed to compare solubiliser (triethanolamine, TEA) or pH modifier (sodium hydroxide)-containing furosemide-loaded electrospun nanofibers from the point of their macro- and microstructural properties during storage.
Methods: Two hydroxypropyl cellulose and poly(vinylpyrrolidone)-based formulations were prepared. Triethanolamine or sodium hydroxide was used for solubility improvement of furosemide. Accelerated stability test (T=40°C, RH=70%, 4 weeks) was performed. Morphological characterisation was carried out by scanning electron microscopy (SEM). X-ray diffraction spectroscopy (XRD) and Fourier-transform infrared spectroscopy (FTIR) were applied to investigate the amorphous or crystalline nature of furosemide in the electrospun samples. Small-volume dissolution test (pH 6.8, 37°C) was worked out to distinguish any difference in the drug release between the two formulations. The furosemide content was determined with UV-vis spectrophotometry at 277 nm wavelength.
Results: The SEM measurements showed similar morphology and fiber diameter distribution in case of the unstored samples. In TEA-containing electrospun samples widened and merged individual fibers appeared from the first week of storage, and along with the time it has become even more dominant. In contrast, the NaOH-containing samples remained the fibrous structure with some slight changes. The microstructural studies confirmed that the formulations contained furosemide in amorphous salt form, and it was preserved during the one-month storage in each case. The dissolution test showed rapid and complete drug release from the fibrous samples.
Conclusion: Amorphous furosemide salt-containing nanofibrous formulations were successfully prepared, which preserved the drug in its amorphous state during the one-month storage. Since the SEM measurements revealed significant differences, the NaOH-containing formulation can be more promising.

Additional Information

Supervisor: Romána Zelkó
E-mail address: zelko.romana@pharma.semmelweis-univ.hu

Bemutatás módja

Szóbeli

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4686

Start

10:25

End

10:40

Authors (legacy)

Andrea Kovács1,2; Adrienn Kazsoki2; Géza Jakab3; Balázs Démuth1,4; Bernadett, Szirányi1; Romána Zelkó2*

1 Gedeon Richter Plc., Formulation R&D, Gyömrői Street 19-21, H-1103 Budapest, Hungary
2 University Pharmacy Department of Pharmacy Administration, Semmelweis University, Hőgyes Endre Street 7-9, H-1092 Budapest
3 Semmelweis University, Department of Pharmaceutics, Hőgyes Endre Street 7, Budapest H-1092, Hungary.

4 Department of Organic Chemistry and Technology, Budapest University of Technology and Economics, Budafoki út 8. 3, H-1103 Budapest, Hungary