PhD Scientific Days 2020

Theoretical and Translational II. Lectures

Microbial Composition and Diversity of Mice Colonized with a Carbapenemase-Producing Klebsiella Pneumoniae Strain

Előadó neve

Farkas, Ferenc Balázs

Előadó munkahelye

Institute of Medical Microbiology

Előadó telefonszáma

+36305644464

Előadó e-mail címe

ferenc.balazs.farkas@gmail.com

Az előadás címe

Microbial Composition and Diversity of Mice Colonized with a Carbapenemase-Producing Klebsiella Pneumoniae Strain

Szerző(k) neve és munkahelye

Ferenc Balázs Farkas1, Judit Domokos1, Dóra Szabó1
1 Institute of Medical Microbiology, Semmelweis University, Budapest

Szekció

Theoretical and Translational II. Lectures

Language of the presentation

English

Section, first choice

Theoretical and Translational Medicine

Section, second choice

Pathology and Oncology

Összefoglaló szövege

Emergence and spread of multidrug-resistant bacteria represent a serious problem worldwide. The extended-spectrum β-lactamase (ESBL) and carbapenemase-producing Enterobacteriaceas (CPE) are in the critical category on the WHO antimicrobial resistance priority list. These bacteria are responsible for a large proportion of hospital-acquired infections. The therapeutic options for CPE are limited, therefore controlling the spread of these bacteria is important. Intestinal colonization plays a crucial role in the spread of CPE.
In this study our goal was to investigate the effect of different per os antibiotic treatments on the microbial composition and diversity of C57BL/6 mice colonized with K. pneumoniae (KP5825). This strain expressed a carbapenemase gene, a PMQR gene and an ESBL gene. We also examined the colonization rate and the resistance gene copy number changes in the colonized mice.
All experiments were carried out using 6–8 weeks-old C57BL/6 male mice. For colonization, 5x10^6 CFU of KP5825 was administered orally on the 14th and 15th days of ampicillin pretreatment. After the colonization mice were divided into 6 groups. These groups received the following antibiotic treatments for 15 days: 0.5g/L ampicillin (AMP), 0.1g/L ceftazidime (CAZ), 0.5g/L ceftazidime, 0.1g/L ciprofloxacin (CIP) and 0.5g/L ciprofloxacin was administered in the drinking water (6th group: control). Fresh fecal pellets were collected from the animals every 3 days throughout the 15 days period. From the fecal samples microbial DNA was extracted.
In the second phase of our experiments we used qPCR technique to determine the KP5825 DNA quantities and assess the resistance gene copy numbers. The V3-V4 region of the 16S rRNA gene was amplified and sequenced with the Illumina MiSeq platform to determine the microbiota composition and diversity in the intestines of colonized mice.
Our results show that AMP and CAZ increased the colonization KP5825 and reduced the gut microbiome diversity originally characterized by Bacteroides dominance. In contrast, CIP did not increase the colonization rate. We also observed strong negative correlation between the presence of Lachnospiraceae NK4A136 group 11319, Roseburia, Anaerostipes, Lachnoclostridium 11308, Lachnospiraceae UCG-004, Tyzzerella, Agathobacter, Lachnospiraceae NK3A20 group 11318 genera and the colonization rate with KP5825.

Additional Information

Supervisor: Dóra Szabó, szabo.dora@med.semmelweis-univ.hu

Research is supported by the ÚNKP-19-2-I-SE-06 New National Excellence Program of the Ministry for Innovation and Technology.

Bemutatás módja

Szóbeli

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4693

Start

12:35

End

12:50

Authors (legacy)

Ferenc Balázs Farkas1, Judit Domokos1, Dóra Szabó1
1 Institute of Medical Microbiology, Semmelweis University, Budapest