PhD Scientific Days 2020

Pathology and Oncology I. Lectures

Population Snapshot of the CTX-M-producing Escherichia Coli Isolated from Haemoculture in a Hungarian Hospital

Előadó neve

Tóth, Kinga, MSc

Előadó munkahelye

Institute of Medical Microbiology, Semmelweis University; Department of Bacteriology, Mycology and Parasitology, National Public Health Center

Előadó telefonszáma

+36302933154

Előadó e-mail címe

kingatoth91@gmail.com

Az előadás címe

Population Snapshot of the CTX-M-producing Escherichia Coli Isolated from Haemoculture in a Hungarian Hospital

Szerző(k) neve és munkahelye

Kinga Tóth1,2, Ákos Tóth1, Ivelina Damjanova1, Katalin Kamotsay3, Viktória Németh3, Dóra Szabó2

1 Department of Bacteriology, Mycology and Parasitology, National Public Health Center, Budapest
2 Institute of Medical Microbiology, Semmelweis University, Budapest
3 Central Microbiology Laboratory, Central Hospital of Southern Pest National Institute of Hematology and Infectious Disease, Budapest

Szekció

Pathology and Oncology I. Lectures

Language of the presentation

English

Section, first choice

Pathology and Oncology

Section, second choice

Molecular Sciences

Összefoglaló szövege

Background: The ST131 high risk clone, especially C2/H30Rx subclade with blaCTX-M-15 and C1-M27 subclade with blaCTX-M-27, are the predominant lineages among third-generation cephalosporin-resistant Escherichia coli. In 2018 44.6% (75/168) of invasive ESBL-producing E. coli isolates investigated at the National Public Health Center belonged to the ST131 clone, where the ratio of C2/H30Rx and C1-M27 was 1 to 0.8. The aim of our study was determine the prevalence of invasive extended-spectrum β-lactamase (ESBL)-producing ST131 E. coli collected from a Hungarian hospital in a short time period in order to compare their genetic backgrounds.
Method: Between October-November 2018, all invasive ESBL-producing E. coli isolates were collected from Central Hospital of Southern Pest. The antimicrobial susceptibility testing was performed according to the EUCAST guidelines. The possible clonal relationships were investigated by core genome (cg)MLST (SeqSphere+) using whole-genome sequencing (WGS) data of isolates obtained from Illumina 251-bp paired-end sequencing. From WGS data acquired antimicrobial resistance and virulence genes were retrieved using ResFinder3.1 and VirulenceFinder2.0 online tools.
Results: From total of six ESBL-producing E.coli isolates five belonged to ST131 clone (two C1-M27 and three C2/H30Rx). The remained one belonged to the ST1193 emerging clone with blaCTX-M-27. Acording to cgMLST all C2/H30Rx isolates showed relatively close clustering (≤6 allele differences), suggested an undetected nosocomial outbreak. The blaCTX-M-27-producing ST1193 isolate and C1-M27 isolates differed at least 35 alleles from each other. All the isolates proved resistant to ceftriaxone and ciprofloxacin, but susceptible to ceftazidim/avibactam, tigecyclin, fosfomycin and carbapenems. C2/H30Rx isolates showed resistance only to ceftazidime, tobramycin and gentamicin. All isolates harboured almost identical virulence gene armament like the adhesion genes (sfa, mat), siderophores (irp, esp) and high invasion associated gene ibe.
Conclusion: This population snapshot analysis highlighted the dominance of the two major CTX-M-producing E. coli subclades regarding invasive infections in Hungary: a currently rising ST131 C1-M27 subclade and the dominant C2/H30Rx one. Only the C2/H30Rx isolates showed close genetic relationship.

Additional Information

Supervisor1: Dóra Szabó
E-mail address: szabo.dora@med.semmelweis-univ.hu
Supervisor2: Ákos Tóth
E-mail address: toth.akos@nnk.gov.hu

Bemutatás módja

Szóbeli

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4697

Start

10:35

End

10:50

Authors (legacy)

Kinga Tóth1,2, Ákos Tóth1, Ivelina Damjanova1, Katalin Kamotsay3, Viktória Németh3, Dóra Szabó2

1 Department of Bacteriology, Mycology and Parasitology, National Public Health Center, Budapest
2 Institute of Medical Microbiology, Semmelweis University, Budapest
3 Central Microbiology Laboratory, Central Hospital of Southern Pest National Institute of Hematology and Infectious Disease, Budapest