PhD Scientific Days 2020

Molecular Sciences I. Posters

Lacking Arhgap25 Racgap Mitigates the Symptoms of Contact Hypersensitivity in Mice

Előadó neve

Czárán, Domonkos, MSc

Előadó munkahelye

Semmelweis University, Department of Physiology

Előadó telefonszáma

+36302643505

Előadó e-mail címe

czaran.domonkos@med.semmelweis-univ.hu

Az előadás címe

Lacking Arhgap25 Racgap Mitigates the Symptoms of Contact Hypersensitivity in Mice

Szerző(k) neve és munkahelye

D. T. Czárán1, R. Pusztai1, K. Ella1, Á. Sűdy1, P. Aradi1, Z. Jakus1, É. Wisniewski1, A. Jobbágy2, Sz. Bozsányi2, K. Lőrincz2, R. Csépányi-Kömi1
1 Semmelweis University, Department of Physiology, Budapest
2 Semmelweis University, Department of Dermatology, Venereology and Dermatooncology

Szekció

Molecular Sciences I. Posters

Language of the presentation

Hungarian

Section, first choice

Molecular Sciences

Section, second choice

Theoretical and Translational Medicine

Összefoglaló szövege

Introduction: Contact hypersensitivity is a complex inflammatory dermal disease mediated by T cells. First contact of the allergen initiates the sensitization phase, which leads to the activation of innate immune cells e.g. neutrophilic granulocytes. Our group previously demonstrated, that the leukocyte specific ARHGAP25 regulates phagocyte functions and has an important role in the effector phase of complex inflammatory diseases.
The aim of this study is to reveal the influence of ARHGAP25 on the pathogenesis of contact hypersensitivity.
Methods: In our experiments either full wild type (WT) and knock out (KO), or bone marrow chimeric mice were used, which either had wild type bone marrow (WT chimeras), or they lacked ARHGAP25 only in the hematopoietic compartment (KO chimeras). For sensitization, the belly of the mice was coated with 3% TNCB (2-chloro-1,3,5-trinitrobenzene), or acetone in the control group. After 5 days, for elicitation, ears were coated with 1% TNCB. Severity of inflammation was investigated by measuring ear thickness, possible tissue damage by examining histological sections, the ratio of different leukocytes was determined by flow cytometry and the composition of cytokine environment by mouse cytokine array.
Results: We observed significantly reduced ear thickening in full KO compered to full WT mice. This difference was also significant between chimeric mice. Neutrophil and cytotoxic T cell count was decreased in the KO ears compared to WT. There was a markable difference in cytokine environment between KO and WT ears. In the control group, no difference was observed, between KO and WT.
Discussion: Our results indicate, that ARHGAP25 expressed in the hematopoietic cells is required for contact hypersensitivity. In its absence the severity of inflammation is significantly lower. This can be the result of the difference in leukocyte and/or cytokine composition between KO and WT ears.
NKFIH FK128376, ÚNKP-19-4-SE-91, Bolyai János Research Grant

Additional Information

Roland Csépányi-Kömi
csepanyi-komi.roland@med.semmelweis-univ.hu

Bemutatás módja

Poszter

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4698

Start

11:29

End

11:32

Authors (legacy)

D. T. Czárán1, R. Pusztai1, K. Ella1, Á. Sűdy1, P. Aradi1, Z. Jakus1, É. Wisniewski1, A. Jobbágy2, Sz. Bozsányi2, K. Lőrincz2, R. Csépányi-Kömi1
1 Semmelweis University, Department of Physiology, Budapest
2 Semmelweis University, Department of Dermatology, Venereology and Dermatooncology