PhD Scientific Days 2020

Pathology and Oncology II. Lectures

Examination of Plasma Cell Subpopulations in Plasma Cell Myeloma

Előadó neve

Czeti, Ágnes, MSc

Előadó munkahelye

1st Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest

Előadó telefonszáma

06209759517

Előadó e-mail címe

czetiagnes@gmail.com

Az előadás címe

Examination of Plasma Cell Subpopulations in Plasma Cell Myeloma

Szerző(k) neve és munkahelye

Ágnes Czeti1, Gábor Szalóki1, Virág Szita2, Ferenc Takács1, Gergely Varga2, Gábor Barna1
1 1st Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest
2 3rd Department of Internal Medicine, Semmelweis University, Budapest

Szekció

Pathology and Oncology II. Lectures

Language of the presentation

Hungarian

Section, first choice

Pathology and Oncology

Section, second choice

Clinical Medicine

Összefoglaló szövege

Introduction: Plasma cell myeloma is an incurable type of cancer. Flow cytometry analysis of bone marrow aspirations plays an important role in its diagnosis and monitoring. A challenge of this method is that immunophenotype of cancer cells can change as a consequence of treatments and antibodies used in immunotherapy can cover epitopes. Thus, new types of plasma cell markers are needed. VS38c is a monoclonal antibody which binds to the CLIMP-63 (Cytoskeleton-Linking Membrane Protein 63) in the endoplasmic reticulum. CLIMP-63 is much more expressed in plasma cells than in other bone marrow cells, thus VS38c could be suitable to separate them.
Aims: We wanted to examine the VS38c staining of normal and neoplastic plasma cells, and whether the preparation of the sample or the time between taking the sample and its processing influence the results. We were also interested in the usability of CLIMP-63 in prognosis.
Method: We examined the bone marrow of 134 myeloma patients with flow cytometry, staining the typical plasma cell surface markers and CLIMP-63. During intracellular staining we used different types and concentrations of permeabilization agents (IntraStain kit, Triton X-100).
Results: We observed two subpopulations of plasma cells while staining with VS38c, a dim and a bright (VS38dim and VS38br). Their ratio depended on the concentration of the permeabilization agent and on how much time passed between taking the sample and its processing. Higher concentration and longer time differences both caused the VS38cdim population to decrease/disappear while VS38cbr increased with the same amount. Washing the sample also led to a loss of the dim population. Furthermore, the VS38cdim population’s ratio increased with the progression of the disease.
Conclusion: VS38c (combined with other markers) is suitable to detect plasma cells. The difference between the populations is probably not caused by difference in expression, but likely by structural differences in the ER membrane. The VS38cdim population is more vulnerable and can be lost during preparation more easily. The change in the ratio of the populations draws attention to that the feasibility of staining the sample also changes during its storage. Furthermore, labeling with VS38c antibody could uncover a myeloma subpopulation which might play an important role in the progression of the disease.

Additional Information

Gábor Barna
barna.gabor@med.semmelweis-univ.hu

Supporting grants:
NVKP_16-1- 2016-0004
EFOP-3.6.3-VEKOP-16-2017-00009

Bemutatás módja

Szóbeli

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4703

Start

11:50

End

12:05

Authors (legacy)

Ágnes Czeti1, Gábor Szalóki1, Virág Szita2, Ferenc Takács1, Gergely Varga2, Gábor Barna1
1 1st Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest
2 3rd Department of Internal Medicine, Semmelweis University, Budapest