PhD Scientific Days 2020

Molecular Sciences II. Posters

Investigation of the Osmoregulatory Role of TRPV4 Ion Channels in Human Podocytes

Előadó neve

Hanyicska, Martin, MSc

Előadó munkahelye

Department of Physiology, Faculty of Medicine - University of Debrecen

Előadó telefonszáma

+36208024123

Előadó e-mail címe

martinhanyicska@science.unideb.hu

Az előadás címe

Investigation of the Osmoregulatory Role of TRPV4 Ion Channels in Human Podocytes

Szerző(k) neve és munkahelye

Martin Hanyicska1,2, Balázs Kelemen1, Erika Lisztes1, Anita Vladár1,2, Márk Racskó1, Tamás Szabó3, Tamás Bíró4,5, István Balázs Tóth1
1 Department of Physiology, Faculty of Medicine - University of Debrecen
2 Doctoral School of Molecular Medicine, University of Debrecen
3 Department of Pediatrics, Faculty of Medicine - University of Debrecen
4 Department of Immunology, Faculty of Medicine - University of Debrecen
5 Hungarian Center of Excellence for Molecular Medicine

Szekció

Molecular Sciences II. Posters

Language of the presentation

Hungarian

Section, first choice

Molecular Sciences

Section, second choice

Theoretical and Translational Medicine

Összefoglaló szövege

The transient receptor potential ion channels (TRP channels), with a few exceptions, have a non-selective permeability to cations, including calcium. These are multimodal cellular sensors that are sensitive to various stimuli in the cell’s physicochemical environment, such as temperature, pH, osmotic pressure, volume, stretch, and vibration. They are expressed in the epithelial cells of the kidney tubules, and our research group discovered thermosensitive TRPV (mainly TRPV4) channels in podocytes, which have an important role in the formation of a glomerular filtration barrier.
In this project we are looking for the role of functionally expressed TRPV4 channels in regulating certain biological processes in podocytes and how they can contribute to the physiological functions of these cells and to the formation of a filtration barrier.
For our experiments we used an immortalized human podocyte cell line. We tested the function of TRPV4 with pharmacological methods by using specific agonist (GSK1016790A) and antagonist (HC067047). We investigated the response of podocytes to hypotonic solutions and the role of TRPV4 susceptible to hypo-osmotic stimuli by using Fluo-4 fluorescent Ca2+ indicator to assess intracellular Ca2+ concentration.
Based on our research, TRPV4 may play an important role in hypotonic stress induced responses, because the intracellular Ca2+ signals evoked by hypo-osmotic challenges were diminished in the presence of the TRPV4 antagonist.
These results suggest that TRPV4 channels in human podocytes could play a role in the regulation of the filtration barrier.

Additional Information

István Balázs Tóth
toth.istvan@med.unideb.hu

Bemutatás módja

Poszter

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4705

Start

12:56

End

12:59

Authors (legacy)

Martin Hanyicska1,2, Balázs Kelemen1, Erika Lisztes1, Anita Vladár1,2, Márk Racskó1, Tamás Szabó3, Tamás Bíró4,5, István Balázs Tóth1
1 Department of Physiology, Faculty of Medicine - University of Debrecen
2 Doctoral School of Molecular Medicine, University of Debrecen
3 Department of Pediatrics, Faculty of Medicine - University of Debrecen
4 Department of Immunology, Faculty of Medicine - University of Debrecen
5 Hungarian Center of Excellence for Molecular Medicine