PhD Scientific Days 2020

Clinical Medicine IV. Lectures

Evaluation of the Expression of Serpin E1/PAI-1 in Biological Samples of Inflammatory Bowel Disease Patients

Előadó neve

Jójárt, Boldizsár, MSc

Előadó munkahelye

University of Szeged, Faculty of Medicine, First Department of Medicine

Előadó telefonszáma

+36309280454

Előadó e-mail címe

jojart.boldizsar@gmail.com

Az előadás címe

Evaluation of the Expression of Serpin E1/PAI-1 in Biological Samples of Inflammatory Bowel Disease Patients

Szerző(k) neve és munkahelye

1 Boldizsár Jójárt University of Szeged, Faculty of Medicine, First Department of Medicine, Hungarian Academy of
Science - University of Szeged Momentum Epithelial Cell Signaling and Secretion Research Group, Szeged
2 Viktória Szabó University of Szeged, Faculty of Medicine, First Department of Medicine, Szeged; Hungarian Academy of Science - University of Szeged Momentum Epithelial Cell Signaling and Secretion Research Group, Szeged
3 Árpád Varga University of Szeged, Faculty of Medicine, First Department of Medicine, Szeged; Hungarian
Academy of Science - University of Szeged Momentum Epithelial Cell Signaling and Secretion Research Group, Szeged
4 Tamás Molnár University of Szeged, Faculty of Medicine, First Department of Medicine, Szeged
5 József Maléth University of Szeged, Faculty of Medicine, First Department of Medicine, Szeged; University of Szeged, Faculty of Medicine, Department of Public Health, Szeged; Hungarian Academy of Science - University of Szeged Momentum Epithelial Cell Signaling and Secretion Research Group, Szeged
6 Klaudia Farkas University of Szeged, Faculty of Medicine, First Department of Medicine, Szeged

Szekció

Clinical Medicine IV. Lectures

Language of the presentation

English

Section, first choice

Molecular Sciences

Section, second choice

Clinical Medicine

Összefoglaló szövege

Introduction: Inflammatory bowel diseases are chronic disabling gastrointestinal disorders. Anti-TNF therapies are primary treatment options in IBD, but in 40-60% of the cases the patients do not respond to the initial treatment or lose response over time. Imbalance of pro-, and anti-inflammatory cytokines alter inflammatory response and determine response to therapy. Thus, patient-specific determination of individual cytokine profiles could improve the prediction of therapeutic response.
Aim: Our aim was to determine the cytokine profile of the IBD patients. In the next step, we wanted to further characterize the expression of promising cytokines.
Methods: Biopsies were obtained from the inflamed and non-inflamed part of the colon of IBD patients and controls undergoing colonoscopy. Total protein and mRNA were isolated from the biopsy samples. We used Cytokine Array to analyse the cytokine pattern. Gene expression and localization of a selected cytokines were assessed by qRT-PCR and immunostaining.
Results: We defined the cytokine profile of 36 biopsy samples. As expected, in the control samples no cytokines were detected, which potentially play a role in the inflammatory process. In samples from IBD patients remarkable discrimination between the inflamed, or non-inflamed areas was possible. MIP1-α/β, IL-1β, IL-8, IL-18 and Serpin E1 were detected in the majority of inflamed samples. Serpin E1/PAI-1 is an inhibitor cytokine inhibiting the tissue plasminogen activator (tPA) resulting in inhibited fibrinolysis and activation of coagulation. As the risk to develop deep venous thrombosis is 6 times higher in the IBD patients than the healthy people, we analysed Serpin E1 further. We compared the expression of Serpin E1 in patients received or not received treatment. Our results suggest that biology therapy decrease the expression of Serpin E1 both at mRNA and protein level.
Conclusion: Our results showed remarkable difference in the cytokine profile of biopsy samples captured from inflamed area compared to controls, or non-inflamed samples. The gene expression of Serpin E1 was the highest in the inflamed sample and the lowest in the control. As a result of biological therapy the expression of SerpinE1 decreased. In the next steps we will increase the sample numbers and analyze the correlation between SerpinE1 expression and clinical response.

Additional Information

József Maléth
jozsefmaleth1@gmail.com

Bemutatás módja

Szóbeli

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4706

Start

18:15

End

18:30

Authors (legacy)

1 Boldizsár Jójárt University of Szeged, Faculty of Medicine, First Department of Medicine, Hungarian Academy of
Science - University of Szeged Momentum Epithelial Cell Signaling and Secretion Research Group, Szeged
2 Viktória Szabó University of Szeged, Faculty of Medicine, First Department of Medicine, Szeged; Hungarian Academy of Science - University of Szeged Momentum Epithelial Cell Signaling and Secretion Research Group, Szeged
3 Árpád Varga University of Szeged, Faculty of Medicine, First Department of Medicine, Szeged; Hungarian
Academy of Science - University of Szeged Momentum Epithelial Cell Signaling and Secretion Research Group, Szeged
4 Tamás Molnár University of Szeged, Faculty of Medicine, First Department of Medicine, Szeged
5 József Maléth University of Szeged, Faculty of Medicine, First Department of Medicine, Szeged; University of Szeged, Faculty of Medicine, Department of Public Health, Szeged; Hungarian Academy of Science - University of Szeged Momentum Epithelial Cell Signaling and Secretion Research Group, Szeged
6 Klaudia Farkas University of Szeged, Faculty of Medicine, First Department of Medicine, Szeged