PhD Scientific Days 2020

Pharmaceutical Sciences I. Posters

Comprehensive Characterisation of Phenolic Profile and Antioxidant Activity of Carpinus Betulus

Előadó neve

Tóth, Csenge Anna

Előadó munkahelye

Department of Pharmacognosy, Semmelweis University

Előadó telefonszáma

06204087137

Előadó e-mail címe

csenge512@gmail.com

Az előadás címe

Comprehensive Characterisation of Phenolic Profile and Antioxidant Activity of Carpinus Betulus

Szerző(k) neve és munkahelye

Csenge Anna Tóth1, Zsófia Garádi1, András Darcsi1, Imre Boldizsár 2, Szabolcs Béni1, Ágnes Alberti1
1 Department of Pharmacognosy, Semmelweis University, Budapest
2 Department of Plant Anatomy, Eötvös Lorand University, Budapest

Szekció

Pharmaceutical Sciences I. Posters

Language of the presentation

Hungarian

Section, first choice

Pharmaceutical Sciences

Section, second choice

Molecular Sciences

Összefoglaló szövege

Introduction: Plants of the Betulaceae family are considered as potential sources of new drugs, however, hornbeam species are rarely explored in this regard. Although phenolic compounds and total antioxidant effect of European hornbeam (Carpinus betulus) leaf methanol extract have been previously evaluated, analysis of other extracts of the plant has not been performed. Additionally, diarylheptanoids bearing remarkable anticancer and antioxidant activity and described in further hornbeam species, have not been identified in C. betulus.
Aims: Our aim was to comprehensively characterise the phenolic profile of C. betulus by analysing extracts prepared from distinct plant parts with various solvents. We devoted particular attention to the possible presence of diarylheptanoids. In addition, we aimed to reveal the contribution of individual constituents to the total antioxidant capacity of the extracts.
Method: Dried and powdered bark, leaf, male and female flower samples of C. betulus were extracted successively with solvents of increasing polarity (chloroform, ethyl acetate, methanol) in ultrasonic bath. For the phytochemical analyses an LC-ESI-MS/MS method was applied. Diarylheptanoids were isolated by combinations of flash chromatographic and semi-preparative HPLC techniques and identified by HR-MS and NMR methods. We analysed the total antioxidant activity of the extracts with the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and the contribution of each compound to the antioxidant effect using an off-line HPLC-DPPH method.
Results: Non-polar compounds in the chloroform extracts were not evaluated. Hydroxycinnamic acids, flavonol-glycosides and diarylheptanoids were representative of ethyl acetate extracts, while methanol extracts were dominated by the presence of gallic acid derivatives and ellagitannins. Five diarylheptanoids were isolated from the bark for the first time. The methanol extract of the leaves showed the highest antioxidant effect, due to its high tannin content. Galloyl-hexahydroxydiphenyl glycosides and other galloyl esters contributed the most to the total antioxidant activity.
Conclusion: Phenolic fingerprints of C. betulus extracts were compared and cyclic diarylheptanoids were identified for the first time. Gallotannins were described as constituents being responsible for the antioxidant activity of hornbeam.
Supported by NKFI K120342.

Additional Information

Ágnes Alberti
email: alberti.agnes@pharma.semmelweis-univ.hu

Bemutatás módja

Poszter

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4711

Start

11:13

End

11:16

Authors (legacy)

Csenge Anna Tóth1, Zsófia Garádi1, András Darcsi1, Imre Boldizsár 2, Szabolcs Béni1, Ágnes Alberti1
1 Department of Pharmacognosy, Semmelweis University, Budapest
2 Department of Plant Anatomy, Eötvös Lorand University, Budapest