PhD Scientific Days 2020

Molecular Sciences IV. Lectures

Whole Genome Analysis of the First Feline Adenovirus Isolate Shows Relationships to Human Adenovirus 1

Előadó neve

Tarcsai, Katalin Réka, MSc

Előadó munkahelye

Department of Medical Microbiology, Semmelweis University, Budapest, Hungary

Előadó telefonszáma

06-30-450-0585

Előadó e-mail címe

tarcsai.katalin-reka@med.semmelweis-univ.hu

Az előadás címe

Whole Genome Analysis of the First Feline Adenovirus Isolate Shows Relationships to Human Adenovirus 1

Szerző(k) neve és munkahelye

Tarcsai, Katalin Réka1, Bányai, Krisztián2, Stercz, Balázs1, Ongrádi, József1
1 Department of Medical Microbiology, Semmelweis University, Budapest
2 Institute for Veterinary Medical Research, Centre for Agricultural Research, Budapest

Szekció

Molecular Sciences IV. Lectures

Language of the presentation

Hungarian

Section, first choice

Molecular Sciences

Section, second choice

Health Sciences

Összefoglaló szövege

Introduction: Adenoviruses transactivate HIV resulting in faster progression of AIDS eliciting opportunistic infections. The feline AIDS model is suitable to study retrovirus-adenovirus interaction, but no adenovirus has been obtained from Felidae. Besides, adenoviruses are used in gene therapy.
Aims: Our aim was to isolate an adenovirus from cats. After epidemiological studies in European and American cats, the feces of seropositive animals was screened for adenovirus nucleic acid by polymerase chain reaction (PCR) and cultivated on human and animal tissue cultures. We isolated an infectious agent. Its biological and molecular characterization, whole genome and phylogenetic analysis were used for identification and establishment its taxonomical order. Its sensitivity to basic environmental effects and antiviral drugs has been tested.
Methods: The cytopathic agent was verified by electron microscopy, immunofluorescence, PCR. Next generation sequencing and phylogenetic analysis using neighbour joining method were carried out. Immunochemistry was used to quantitate infectivity following in vitro treatments.
Results: The infectious agent proved to be an adenovirus and named feline adenovirus (FeAdV). Unusually, it replicates in several human and animal cell lines. Next generation sequencing identified its genome consisting of 35898 nucleotides. Phylogenetic analysis showed that FeAdV is related to human adenovirus (HAdV)-1 containing 36001 nucleotides. One nucleotide difference was demonstrated between FeAdV and HAdV-1 at position of 15081 affecting the central region of L3 gene (penton) without amino acid change. As compared to adenoviruses, FeAdV shows similar sensitivity to UV, higher sensitivity to heat, detergents and antiviral drugs, but more resistant to chlorine.
Conclusions: Relatedness of FeAdV to HAdV-1 raises the possibility of a zoonotic infection, although the human and feline pathogenesis has not been revealed. Deletion of the first and last 6 nucleotides in FeAdV might explain different biological properties and wide host range contrary to species specificity of adenoviruses. This might be based on the altered surface structure of viral polypeptides binding to cell receptors. Recombinant human and animal adenovirus are used as vectors, but due to biological incongruences new adenoviruses as FeAdV would be ideal candidates for new vectors.

Additional Information

Supervisor: József Ongrádi
E-mail address: ongradi.jozsef@med.semmelweis-univ.hu

Bemutatás módja

Szóbeli

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4719

Start

12:25

End

12:40

Authors (legacy)

Tarcsai, Katalin Réka1, Bányai, Krisztián2, Stercz, Balázs1, Ongrádi, József1
1 Department of Medical Microbiology, Semmelweis University, Budapest
2 Institute for Veterinary Medical Research, Centre for Agricultural Research, Budapest