PhD Scientific Days 2020

Molecular Sciences I. Posters

Lymphatics Modulate the Development of Contact Hypersensitivity

Előadó neve

Aradi, Petra, MSc

Előadó munkahelye

Department of Physiology, Semmelweis University School of Medicine, Budapest, Hungary

Előadó telefonszáma

+36 20 396 37 90

Előadó e-mail címe

aradi.petra@med.semmelweis-univ.hu

Az előadás címe

Lymphatics Modulate the Development of Contact Hypersensitivity

Szerző(k) neve és munkahelye

Petra Aradi1,2, Éva Kemecsei1,2, Gábor Kovács1,2 and Zoltán Jakus1,2
1 Department of Physiology, Semmelweis University School of Medicine, Budapest, Hungary
2 MTA-SE „Lendület” Lymphatic Physiology Research Group of the Hungarian Academy of Sciences and the Semmelweis University, Budapest, Hungary

Szekció

Molecular Sciences I. Posters

Language of the presentation

Hungarian

Section, first choice

Molecular Sciences

Section, second choice

Theoretical and Translational Medicine

Összefoglaló szövege

Introduction: Contact hypersensitivity (CHS) reaction, the mouse model of human allergic contact dermatitis, is inducible by repeated exposure to contact allergens. In the sensitization phase immune cells are activated by allergens, while in the elicitation phase activated immune cells induce inflammation and tissue destruction following the second antigen exposure. Lymphatics play an important role in the regulation of the immune response in infectious diseases of the skin but the function of lymphatics in the development of allergic contact dermatitis remains unclear.

Aim: We aimed to characterize the role of the lymphatics in sensitization and elicitation phases of contact hypersensitivity.

Methods: In this study Vegfr3kd/+ mice, which lack lymphatics in the entire skin, and a conditional lymphatic loss of function mouse model, in which local deletion of the lymphatic vessels can be induced by diphtheria toxin (DT) injection were used. CHS was initiated by the exposure of the skin to TNCB followed by a second treatment. Disease progression was assessed by ear thickness measurements, hematoxylin and eosin histology, and immunostaining of lymphatic and immune cell markers. The infiltrated immune cells were monitored by flow cytometry.

Results: Our results revealed reduced inflammation with reduced immune cell infiltration was detected in Vegfr3kd/+ mice lacking lymphatics both in the sensitization and the elicitation phases compared to the control. In contrast, DT-injected mice lacking lymphatics only in the elicitation phase showed robust inflammation. In the adaptive CHS model sensitized immune cells from wild type mice were transferred into Vegfr3kd/mice lacking lymphatics in the skin, and increased inflammation with more infiltrating immune cells was detected compared to the control.

Conclusion: We conclude that skin lymphatics play an important role in the sensitization and elicitation phases of CHS, however their function is different in the two phases. Lymphatics contribute to the sensitization process of the immune cells in the sensitization phase, while they moderate the inflammatory response and immune cell infiltration in the elicitation phase.

Additional Information

Supervisor: Zoltán Jakus
E-mail address: jakus.zoltan@med.semmelweis-univ.hu

Bemutatás módja

Poszter

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

2701

Start

11:20

End

11:23

Authors (legacy)

Petra Aradi1,2, Éva Kemecsei1,2, Gábor Kovács1,2 and Zoltán Jakus1,2
1 Department of Physiology, Semmelweis University School of Medicine, Budapest, Hungary
2 MTA-SE „Lendület” Lymphatic Physiology Research Group of the Hungarian Academy of Sciences and the Semmelweis University, Budapest, Hungary