PhD Scientific Days 2020

Theoretical and Translational V. Lectures

Association Between Gut Microbial Diversity and Various Cardiovascular Imaging Phenotypes

Előadó neve

Dr. Helga, Szabó

Előadó munkahelye

Medical Imaging Centre, Semmelweis University

Előadó telefonszáma

+36202350674

Előadó e-mail címe

szabo.helga.se@gmail.com

Az előadás címe

Association Between Gut Microbial Diversity and Various Cardiovascular Imaging Phenotypes

Szerző(k) neve és munkahelye

Helga Szabo1, Nora Makra2, Peter Fussy3, Luca Zoldi3, Anita Hernyes1, David Laszlo Tarnoki1,4, Dora Szabo2, Adam Domonkos Tarnoki1,4
1 Medical Imaging Centre, Semmelweis University, Budapest, Hungary
2 Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary
3 Faculty of Medicine, Semmelweis University, Budapest, Hungary
4 Department of Radiology, Oncologic Imaging Diagnostic Center, National Institute of Oncology, Budapest, Hungary

Szekció

Theoretical and Translational V. Lectures

Language of the presentation

English

Section, first choice

Theoretical and Translational Medicine

Section, second choice

Clinical Medicine

Összefoglaló szövege

Introduction: The importance of the gut microbiome is growing, because the composition of the gastrointestinal microbiome greatly influences the development, process and therapy of diseases. The role of lifestyle in this process is unclear, as well as how it modifies the heritability of the diseases studied.
Aims: To find the associations between intestinal microbiome diversity, environmental factors and various cardiovascular imaging phenotypes.
Method: 114 twins (mean age 53.4±14.4 years, 59% female, 55 monozygotic, MZ and 2 same-sex dizygotic, DZ pairs) recruited from the Hungarian Twin Registry were involved who met at least one of the following criteria: (1) carotid and femoral ultrasound examination revealed increased intima-media thickness (IMT) or plaque; (2) twins discordant for obstructive sleep apnea (OSA), osteoporosis, fatty liver, thyroid nodule, cervical or lumbar discus bulging or hernia; (3) MZ twins diagnosed with Hashimoto thyreoiditis or OSA. A special stool sampling container was mailed and received from each participant. After DNA extraction, library construction was performed specifically for the V3-V4 hypervariable region of microbial 16S rDNA. Next, the microbiome composition of the samples was determined using the QIIME software. In addition, questionnaires were completed to assess health status, dietary habits and physical activity. After getting all the data a comparison with the various cardiovascular imaging phenotypes, including local arterial stiffness, IMT and plaque burden will be possible.
Results: Until the end of January 2020, 100 participants returned their stool samples from 34 cities throughout the country, mainly (56 subjects) from Budapest. The youngest twin pair was 25 and the oldest was 76 years old. BMI of the participants proved to be variable (mean 26±4.5 kg/m2). 13% of the twins smoked, 46% have been diagnosed with hypertension and 6% with diabetes. All the twins with OSA (48 subjects) and 93% of twins having increased IMT or plaque burden returned their samples.
Conclusion: This is the first Hungarian twin study to assess gut microbiome in twins. By mapping the gastrointestinal microbiome, we can gain a more accurate picture how the composition and interactions of the microbiome can influence the atherosclerotic imaging phenotypes. Besides, more effective prevention strategies and treatments can be developed.

Additional Information

Supervisors: David Laszlo Tarnoki & Adam Domonkos Tarnoki

Bemutatás módja

Szóbeli

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4070

Start

17:30

End

17:45

Authors (legacy)

Helga Szabo1, Nora Makra2, Peter Fussy3, Luca Zoldi3, Anita Hernyes1, David Laszlo Tarnoki1,4, Dora Szabo2, Adam Domonkos Tarnoki1,4
1 Medical Imaging Centre, Semmelweis University, Budapest, Hungary
2 Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary
3 Faculty of Medicine, Semmelweis University, Budapest, Hungary
4 Department of Radiology, Oncologic Imaging Diagnostic Center, National Institute of Oncology, Budapest, Hungary