PhD Scientific Days 2020

Molecular Sciences II. Posters

Examination of Serum miRNAs as Possible Biomarkers for the Diagnosis of Acute Rejection after Kidney Transplantation

Előadó neve

Dr. Janszky, Noémi

Előadó munkahelye

3rd Department of Internal Medicine, Semmelweis University, Budapest, Hungary

Előadó telefonszáma

06303670378

Előadó e-mail címe

janszkynoemi@gmail.com

Az előadás címe

Examination of Serum miRNAs as Possible Biomarkers for the Diagnosis of Acute Rejection after Kidney Transplantation

Szerző(k) neve és munkahelye

Noémi Janszky1, Ágnes Szilágyi1, Csuka Dorottya1, Claudia Sommerer2, Christian Morath2, Güclü Aykut2, Martin Zeier2, Prohászka Zoltán1, Caner Süsal2

1 3rd Department of Internal Medicine, Semmelweis University, Budapest, Hungary

2 Transplantation Immunology, Institute of Immunology, University of Heidelberg, Heidelberg, Germany; Department of Nephrology, University of Heidelberg, Heidelberg, Germany

Szekció

Molecular Sciences II. Posters

Language of the presentation

Hungarian

Section, first choice

Molecular Sciences

Section, second choice

Molecular Sciences

Összefoglaló szövege

Introduction: Because of the lack of non-invasive, specific biomarkers, early detection of acute rejection after kidney transplantation represents a challenge. microRNAs (miRNAs) belong to a class of small, noncoding RNAs and they were found to be involved in pathological processes that occur following kidney transplantation.
Aims: We analyzed the association of serum expression levels of miR-15b, miR-16, miR-24, miR-103a and miR-107 with acute rejection episodes that occur during the early phase after transplantation. We also investigated the capability of these miRNAs to differentiate between acute rejection episodes and other pathological processes.
Patients and Methods: Prospectively collected serum samples of kidney allograft recipients with stable graft function (n=10), urinary tract infection (UTI) (n=9), borderline rejection (n=9), acute tubular necrosis (ATN) (n=9) or biopsyproven acute rejection (n=9), who were transplanted between 2015 and 2017 were analyzed. In addition, serum samples of 7 patients with an acute rejection episode were also investigated at different time points after kidney transplantation. miRNA expression levels were determined by real-time quantitative reverse-transcription-polymerase chain reaction (RT-PCR). Spiked-in cel-miR-39 was used as a normalization control.
Results and Discussion: As compared to patients with stable graft function, decreased serum levels of miR-15b, miR-16 and miR-24 were observed on post-Tx day 8 in kidney transplant patients with borderline rejection, ATN or acute rejection. miR-15b and miR-24 levels were lower in patients with ATN than in patients with UTI. miR-103a level was also lower in patients with ATN or acute rejection than in patients with stable function. Longitudinal analysis of individual patients showed that miR-107 is increased during acute rejection and decreases with the improvement of the kidney function. One patient with ABMR showed extremely decreased levels at all timepoints.
Conclusion: The determination of serum miR-15b, miR-16, miR-24 and miR-103a levels may allow the detection of allograft damage after kidney transplantation; however, these miRNAs do not seem to distinguish acute rejection from other transplant pathologies. In contrast, the quantification of serum miR-107 appears to be useful for monitoring the therapy success in patients with an acute rejection episode.

Additional Information

Dorottya Csuka
csukadorka@gmail.com

Bemutatás módja

Poszter

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4164

Start

13:02

End

13:05

Authors (legacy)

Noémi Janszky1, Ágnes Szilágyi1, Csuka Dorottya1, Claudia Sommerer2, Christian Morath2, Güclü Aykut2, Martin Zeier2, Prohászka Zoltán1, Caner Süsal2

1 3rd Department of Internal Medicine, Semmelweis University, Budapest, Hungary

2 Transplantation Immunology, Institute of Immunology, University of Heidelberg, Heidelberg, Germany; Department of Nephrology, University of Heidelberg, Heidelberg, Germany