PhD Scientific Days 2020

Pharmaceutical Sciences II. Lectures

Occurrence of Bacteria Producing Extended Spectrum β-lactamases in Food Animals

Előadó neve

Balázs, Bence, MSc

Előadó munkahelye

Department of Medical Microbiology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary

Előadó telefonszáma

+36702461349

Előadó e-mail címe

balazsbence0903@gmail.com

Az előadás címe

Occurrence of Bacteria Producing Extended Spectrum β-lactamases in Food Animals

Szerző(k) neve és munkahelye

Bence Balázs1;2, József Bálint Nagy1;2, Zoltán Tóth1;2, Fruzsina Nagy1;2, Turcsányi Ibolya3, Bistyák Andrea3, Gábor Kardos1

1 Department of Medical Microbiology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary
2 Doctoral School of Pharmaceutical Sciences, University of Debrecen, Debrecen, Hungary
3 Debrecen Laboratory, Veterinary Diagnostic Directorate, National Food Chain Safety Office, Debrecen, Hungary

Szekció

Pharmaceutical Sciences II. Lectures

Language of the presentation

Hungarian

Section, first choice

Pharmaceutical Sciences

Section, second choice

Health Sciences

Összefoglaló szövege

Multidrug resistance due to production of extended-spectrum beta-lactamases (ESBLs) is a major problem in human as well as veterinary medicine. These strains appear in animal and human microbiome and can be the source of infection both in animal and human healthcare, in accordance with the One Health theorem. In this study we examined the prevalence of ESBL producing bacteria in food animals.

We examined 100 porcine and 114 poultry faecal samples. The samples were cultured on eosine-methylene-blue agar supplemented with 2 mg/L cefotaxime, recovered isolates were identified by MALDI-TOF-MS. Susceptibility testing was performed by Kirby-Bauer disk-diffusion test based on EUCAST guidelines; ESBL phenotype was examined using the double-disk method.

In the porcine and poultry samples 72 and 39 ESBL-producing isolates were found, respectively. All poultry and 43 porcine isolates were Escherichia coli; 21 Proteus spp., 7 Myroides odoratimimus, 5 Citrobacter freundii, 2 Morganella morganii and 1 Providencia rettgeri were also found in porcine isolates. We compared further E. coli of poultry and porcine origin.

The phenotypic resistance was similar among the porcine and poultry E. coli isolates: amoxicillin/clavulanic acid (79,1% vs. 74,1%), cefotaxime (100,0% vs. 99,0%), cefepime (97,7% vs. 97,5%), colistin (0,0% vs. 2,6%), amikacin (39,5% vs. 35,9%) and tobramycin (39,5% vs. 35,9%). In case of gentamicin and sulfamethoxazole/trimethoprim (SXT) the resistance was higher within porcine isolates (79,1% vs. 12,8% and 81,4% vs. 35,9%, respectively). All isolates were susceptible to carbapenems.
The prevalence of ESBL gene families were different among the two type of isolates. The most common was group CTX-M-1, its prevalence was higher in porcine isolates (90,7% vs. 69,7%). The CTX-M-2 gene family found only in porcine isolates (4,7%), while the CTX-M-9 have been found in case of poultry E. coli (7,7%). CTX-M-8 family was totally absent.

In summary, the ESBL producing bacteria are prevalent in the faecal samples of the examined food animals, with a dominance of the CTX-M-1 group enzymes.

Additional Information

Bence Balázs, Zoltán Tóth, Fruzsina Nagy were supported by New National Excellence Program of the Ministry for Innovation and Technology (ÚNKP-19-3-I.)
Bence Balázs, Zoltán Tóth, József Bálint Nagy were supported by EFOP-3.6.3-VEKOP-16-2017-00009

Supervisor: Gábor Kardos
E-mail: kg@med.unideb.hu

Bemutatás módja

Szóbeli

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4732

Start

11:55

End

12:10

Authors (legacy)

Bence Balázs1;2, József Bálint Nagy1;2, Zoltán Tóth1;2, Fruzsina Nagy1;2, Turcsányi Ibolya3, Bistyák Andrea3, Gábor Kardos1

1 Department of Medical Microbiology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary
2 Doctoral School of Pharmaceutical Sciences, University of Debrecen, Debrecen, Hungary
3 Debrecen Laboratory, Veterinary Diagnostic Directorate, National Food Chain Safety Office, Debrecen, Hungary