PhD Scientific Days 2020

Health Sciences Posters

Studying the Proteinase Complex of Micromycete Tolypocladium Inflatum as a New Approach for Thrombotherapy

Előadó neve

Fokichev, Nikolai

Előadó munkahelye

Lomonosov Moscow State University (MSU)

Előadó telefonszáma

+79814220912

Előadó e-mail címe

fokichev.n@mail.ru

Az előadás címe

Studying the Proteinase Complex of Micromycete Tolypocladium Inflatum as a New Approach for Thrombotherapy

Szerző(k) neve és munkahelye

Nikolai S. Fokichev1, Kornienko I. Elena1, Osmolovskiy A. Alexander1
1 Lomonosov Moscow State University (MSU), Moscow

Szekció

Health Sciences Posters

Language of the presentation

English

Section, first choice

Health Sciences

Section, second choice

Molecular Sciences

Összefoglaló szövege

Introduction
The problem of fibrin clots and thrombosis prevention during the severe cardio-vascular disorders such as infarctions, ischemic strokes and blood diseases remains extremely relevant for vascular medicine. Along with already known methods of treatment, new approaches are emerging that involve the use of micromycete proteinases to prevent thrombosis.

Aims
To examine the biochemical properties, defining the possible therapeutic application of micromycete T.inflatum.

Method
The preparation of proteinases was obtained by culture fluid proteins precipitation with acetone, followed by drying stage on the sixth day of cultivation, performed with thermostatically controlled shaker in a deep culture conditions on a selected medium. The protein fractions separation of the drug was conducted by the method of liquid isoelectric focusing with the activity control by caseinolysis. Proteolytic and plasminogen activator activity was measured by fibrin plates method. Thrombolytic potential was examined in a fibrin thrombus lysis model (evaluation the weight loss of fibrin clot after 30 min, 1hr and 3 hrs exposition with the preparation of proteinases).

Results
After isoelectrofocusing were obtained 3 peak fractions with the caseinolysis activities 77,4; 111,9 and 179,8 mkM Tyr/ml×min. The fibrinolytic activities were 333; 255 and 934 u/ml respectively and the plasminogen activator activities were 169,3; 163,5 and 328 respectively, which is comparable to well-known plasminogen activators (e.g. urokinase) or preparations, obtained from investigated plasminogen activators, such as Sarocladium strictum.In experiments with the fibrin clot lysis model the preparation of T. inflatum proteinases (fractions all together ) demonstrated high efficiency of fibrin clot thrombolysis (29,6 % of fibrin clot weight elimination after 30 min; 70,1 % after 1 hr; 90,0% after 3hrs.)

Conclusion
The obtained data indicate that the proteinases preparation, obtained from the culture fluid of the micromycete Tolypociadium inflatum, demonstrates the pronounced thrombolytic, fibrinolytic and plasminogen activator activities, which allows to suggest the therapeutical potential in thrombosis treatment in vascular medicine and, what is more, the biotechnological potential for the development of diagnostic kit for hemostasis system disorders monitoring.

Additional Information

Supervisor: Alexander Osmolovskiy
E-mail address: aosmol@mail.ru

Bemutatás módja

Poszter

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4734

Start

11:23

End

11:26

Authors (legacy)

Nikolai S. Fokichev1, Kornienko I. Elena1, Osmolovskiy A. Alexander1
1 Lomonosov Moscow State University (MSU), Moscow