PhD Scientific Days 2020

Neurosciences I. Lectures

Anatomical Mapping of Cariprazine Binding Sites in the Brain

Előadó neve

Dr. Prokop, Susanne

Előadó munkahelye

Institute of Experimental Medicine, Budapest, Hungary

Előadó telefonszáma

06308308770

Előadó e-mail címe

prokop.susanne@koki.mta.hu

Az előadás címe

Anatomical Mapping of Cariprazine Binding Sites in the Brain

Szerző(k) neve és munkahelye

Susanne Prokop1, Benjamin Barti1, Marton Vamosi1, Gabriella Urban1, Attila Egyed2, Gyorgy M. Keseru2, Istvan Katona1

1 Institute of Experimental Medicine, Budapest, Hungary
3 Research Centre for Natural Sciences, Budapest, Hungary

Szekció

Neurosciences I. Lectures

Language of the presentation

English

Section, first choice

Neurosciences

Section, second choice

Neurosciences

Összefoglaló szövege

Cariprazine is a new third generation antipsychotic drug, used in the treatment of scizophrenia, major depression and bipolar disorder. Despite of its clinical success, the underlying mechanism of its action remains elusive. The exploration of the exact anatomical localization of cariprazine binding sites in the brain can improve our understanding of effects of cariprazine, and it may aid the development of new drugs acting in the central nervous system.
We aimed to generate a map of high affinity cariprazine binding sites in the brain. The application of immunohistochemical markers allows the identification of specific brain regions, cell types and cellular profiles, that are occupied by the drug. To reveal the nanoscale distribution of the drug, we also aimed to perform super-resolution imaging.
To investigate the large-scale three-dimensional distribution of the drug, we registered and reconstructed epifluorescent image sequences of histological slices. For more detailed examination, we generated confocal microscopic and stochastic optical reconstruction microscopy (STORM) super-resolution images. Brain samples from wild type and D3 dopamine receptor knock-out animals were investigated. We found a unique distribution of cariprazine binding sites in the forebrain, which is due to its preference towards D3 dopamine receptor.
In summary, our results reveal new insights into the molecular and anatomical basis of cariprazine actions.
SUPPORTED BY THE ÚNKP-19-3-I-SE-55 NEW NATIONAL EXCELLENCE PROGRAM OF THE MINISTRY FOR INNOVATION AND TECHNOLOGY.

Additional Information

Supervisor: Istvan Katona
katona@koki.hu

Bemutatás módja

Szóbeli

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4090

Start

12:05

End

12:20

Authors (legacy)

Susanne Prokop1, Benjamin Barti1, Marton Vamosi1, Gabriella Urban1, Attila Egyed2, Gyorgy M. Keseru2, Istvan Katona1

1 Institute of Experimental Medicine, Budapest, Hungary
3 Research Centre for Natural Sciences, Budapest, Hungary