PhD Scientific Days 2020

Molecular Sciences III. Posters

The Effect of High-NaCl Intake on Skin Tissue Remodeling

Előadó neve

Dr. Pajtók, Csenge

Előadó munkahelye

1st Department of Paediatrics, Semmelweis University, Budapest

Előadó telefonszáma

+36306543170

Előadó e-mail címe

pajtok.csenge@gmail.com

Az előadás címe

The Effect of High-NaCl Intake on Skin Tissue Remodeling

Szerző(k) neve és munkahelye

Csenge Pajtók1, Domonkos Pap1,2, Róbert Agócs1, Dániel András Sugár1, Apor Veres-Székely1, István Németh3, Zoltán Veréb3, Lajos Kemény3, Ádám Vannay1,2, Attila J. Szabó1,2, Tivadar Tulassay1,2

1 1st Department of Paediatrics, Semmelweis University, Budapest
2 MTA-SE Pediatrics and Nephrology Research Group, Budapest
3 Department of Dermatology and Allergology, Faculty of Medicine, University of Szeged

Szekció

Molecular Sciences III. Posters

Language of the presentation

English

Section, first choice

Molecular Sciences

Section, second choice

Clinical Medicine

Összefoglaló szövege

Introduction: Wound healing is a complex, inflammation and extracellular matrix (ECM) remodeling associated process in which dermal fibroblasts play a central role. Based on literature data, high-NaCl intake induces inflammatory response and causes or often worsens several pathological conditions. However, the effect of high-NaCl diet on the process of skin ECM remodeling and fibroblast activity is not fully understood.
Methods: To investigate the effect of high-NaCl consumption on skin tissue remodeling we kept the mice on high NaCl diet and than we performed the imiquimod (IMQ) induced mouse model of dermatitis. Dermal mRNA level of ECM component collagen-1, fibronectin and the fibroblast marker alpha smooth muscle actin (α-SMA) was measured by real-time RT-PCR. The effect of increased NaCl concentration on ECM production and cellular motility was investigated on human primary dermal fibroblasts in vitro.
Results: The effect of high-NaCl diet on the IMQ treated group resulted in lower skin mRNA levels of fibronectin, collagen1 and α-SMA compared to the IMQ treated mice on normal salt diet. Increased NaCl concentration reduced the fibronectin and collagen-1 mRNA expression, as well as the TGF-β induced fibroblast motility in human dermal fibroblast cell line.
Conclusion: Taken together, our results reveal that high salt intake reduces the expression of ECM markers and motility of dermal fibroblasts. Therefore high dietary NaCl intake may be responsible for hindered wound healing.

Grant support: This paper was supported 20382-3/2018 FEKUTSTRAT grant, OTKA K125470 and Semmelweis Science and Innovation Fund.

Additional Information

Supervisor: Domonkos Pap
e-mail address: pap.doma@gmail.com

Bemutatás módja

Poszter

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4740

Start

11:19

End

11:22

Authors (legacy)

Csenge Pajtók1, Domonkos Pap1,2, Róbert Agócs1, Dániel András Sugár1, Apor Veres-Székely1, István Németh3, Zoltán Veréb3, Lajos Kemény3, Ádám Vannay1,2, Attila J. Szabó1,2, Tivadar Tulassay1,2

1 1st Department of Paediatrics, Semmelweis University, Budapest
2 MTA-SE Pediatrics and Nephrology Research Group, Budapest
3 Department of Dermatology and Allergology, Faculty of Medicine, University of Szeged