PhD Scientific Days 2020

Molecular Sciences III. Posters

Effect of Ethanol Exposure on Longevity and Chemotaxis on Caenorhabditis Elegans

Előadó neve

Körmendi, Petra

Előadó munkahelye

Department of Medical Chemistry, Molecular Biology and Pathobiochemistry

Előadó telefonszáma

+36302495687

Előadó e-mail címe

petra.bianka.kormendi@gmail.com

Az előadás címe

Effect of Ethanol Exposure on Longevity and Chemotaxis on Caenorhabditis Elegans

Szerző(k) neve és munkahelye

Körmendi Petra, Cynthia Hunn, Gábor Hajdú, and Csaba Barta
Semmelweis University
Department of Medical Chemistry, Molecular Biology and Pathobiochemistry

Szekció

Molecular Sciences III. Posters

Language of the presentation

Hungarian

Section, first choice

Molecular Sciences

Section, second choice

Neurosciences

Összefoglaló szövege

Alcohol is one of the most frequently abused addictive substances in humans, which has a wide range of effects on the central nervous system. About 70% of C. elegans genes are homologous to those of humans and a resemblance between the nervous systems of the two species can clearly be pointed out: Dopaminergic as well as serotonergic neurons are present in their central nervous systems, which play a role in the reward system and are indispensable when discussing addiction. Both dopamine and serotonin affect movement and behavior of the animals. The fully mapped out neuronal network and the precise knowledge of the functionality of its synapses makes C. elegans a valuable model for addiction research.
In the longevity paradigm C. elegans was treated with 400mM and 200mM ethanol from day one adult age onwards. In the diacetyl race paradigm animals were conditioned to 400mM and 200mM ethanol for 24 hours during late L4-young adult state, and after 1 hour withdrawal period transferred to a race plate (infused with or without ethanol) and subjected to an attractive odorant stimulus (diacetyl).
High dose ethanol reduces lifespan by almost 50%, whereas low dose has either no, or considerably effect on longevity. Upon alcohol treatment, the worm’s characteristic movements are also altered. In diacetyl race after high dose treatment (400mM), the worms may lose the ability to coordinate their movement, orientation or their interest towards the attractant diacetyl. Upon lower dosage, these responses were less prevalent. Wild type (N2) animals were compared to serotonin deficient (tryptophane-hydroxylase) tph-1-, and dopamine receptor deficient dop-2 (ortholog of human DRD2) null mutants.
Our results have shown that alcohol induces changes in C. elegans longevity as well as behavior in a dose dependent manner. High concentration ethanol exposure decreases life span dramatically and hinders locomotor performance that can be at least partially rescued by low dose exposure during the race. Neurotransmitter deficient mutants also showed alterations in motility patterns. A further advantage of the worm model is the possibility of examining transgenerational and epigenetical effects.

Additional Information

Supervisor:
Barta Csaba
barta.csaba@med.semmelweis-univ.hu

Bemutatás módja

Poszter

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4752

Start

11:10

End

11:13

Authors (legacy)

Körmendi Petra, Cynthia Hunn, Gábor Hajdú, and Csaba Barta
Semmelweis University
Department of Medical Chemistry, Molecular Biology and Pathobiochemistry