PhD Scientific Days 2020

Molecular Sciences IV. Posters

Species-Specific Glucose-6-Phosphatase Activity in the Small Intestine—Studies in Three Different Mammalian Models

Előadó neve

Varga, Viola, MSc

Előadó munkahelye

Transzlációs Medicina Intézet

Előadó telefonszáma

705309200

Előadó e-mail címe

varviola@gmail.com

Az előadás címe

Species-Specific Glucose-6-Phosphatase Activity in the Small Intestine—Studies in Three Different Mammalian Models

Szerző(k) neve és munkahelye

Viola Varga 1 , Zsófia Murányi 1 , Anita Kurucz 2 , Paola Marcolongo 3 , Angelo Benedetti 3 , Gábor Bánhegyi 2,4 and Éva Margittai 1,*
1 Institute of Translational Medicine, Semmelweis University, 1094 Budapest, Hungary; varga.viola@med.semmelweis-univ.hu (V.V.); sophie0924@freemail.hu (Z.M.)
2 Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University, 1094 Budapest, Hungary; kurucz.anita.andrea@gmail.com (A.K.); banhegyi@eok.sote.hu (G.B.)
3 Department of Molecular and Developmental Medicine, University of Siena, 53100 Siena, Italy; paola.marcolongo@unisi.it (P.M.); benedetti@unisi.it (A.B.)
4 Pathobiochemistry Research Group of Hungarian Academy of Sciences & Semmelweis University, 1094 Budapest, Hungary

Szekció

Molecular Sciences IV. Posters

Language of the presentation

English

Section, first choice

Molecular Sciences

Section, second choice

Health Sciences

Összefoglaló szövege

The liver is a well-known gluconeogenic organ responsible for the maintenance of blood glucose level in all post-absorptive situations. However, kidneys and intestines can also produce glucose in blood, particularly during fasting and under protein feeding. The major enzyme involved is the glucose-6-phosphatase, which catalyzes the common terminal reaction of gluconeogenesis and glycogenolysis, i.e., the hydrolysis of glucose-6-phosphate to glucose and inorganic phosphate. Observations gained in different experimental animals have given ambiguous results concerning the activity and presence of the glucose-6-phosphatase system in the small intestine. The aim of this study was to better define the species-related differences of this putative gluconeogenic organ in glucose homeostasis. The components of the glucose-6-phosphatase system (i.e., glucose-6-phosphate transporter and glucose-6-phosphatase itself) were analyzed at mRNA and protein level in the small intestine mucosae and liver of rats, guinea pigs, and humans. Glucose-6-phosphatase activities were also detected. The results showed that the glucose-6-phosphatase system is poorly represented in the small intestine of rats; on the other hand, significant expressions of glucose-6-phosphate transporter and of the glucose-6-phosphatase were found in the small intestine of guinea pigs and humans. The activity of the recently described fructose-6-phosphate transporter–intraluminal hexose isomerase pathway was also present in intestinal microsomes from these two species. The results demonstrate that the gluconeogenic role of the small intestine is highly species-specific and presumably dependent on feeding behaviour (e.g., fructose consumption) and the actual state of metabolism.
Keywords: glucose-6-phosphatase; glucose-6-phosphate transporter; small intestine; endoplasmic reticulum; fructose

Additional Information

Supervisor: Éva Margittai
Email address: margittai.eva@med.semmelweis-univ.hu

Bemutatás módja

Poszter

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

4753

Start

12:43

End

12:46

Authors (legacy)

Viola Varga 1 , Zsófia Murányi 1 , Anita Kurucz 2 , Paola Marcolongo 3 , Angelo Benedetti 3 , Gábor Bánhegyi 2,4 and Éva Margittai 1,*
1 Institute of Translational Medicine, Semmelweis University, 1094 Budapest, Hungary; varga.viola@med.semmelweis-univ.hu (V.V.); sophie0924@freemail.hu (Z.M.)
2 Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University, 1094 Budapest, Hungary; kurucz.anita.andrea@gmail.com (A.K.); banhegyi@eok.sote.hu (G.B.)
3 Department of Molecular and Developmental Medicine, University of Siena, 53100 Siena, Italy; paola.marcolongo@unisi.it (P.M.); benedetti@unisi.it (A.B.)
4 Pathobiochemistry Research Group of Hungarian Academy of Sciences & Semmelweis University, 1094 Budapest, Hungary